Abstract
Jerusalem artichoke is a valuable source of inulin, a polysaccharide. Inulin is a sustainable source of dietary fiber that enhances the immune system in humans. Although Jerusalem artichoke can be propagated vegetatively, breeders use cross-fertilization to produce novel varieties and hybrids with higher inulin yields. Seed dormancy can hinder the breeding progress because dormancy reduces the number of generations a breeder can obtain in 1 year. Current methods for breaking seed dormancy are time consuming and usually involve removing or pin-pricking the seed coat of these very small seeds, or using several weeks of seed vernalization or several months of storage. Breeders thus require better methods that are less time consuming and achieve higher germination percentages. Here, we studied germination, dormant seed, dead seed, and the seedling growth rate of different Jerusalem artichoke genotypes and seed lots after seeds were treated with different methods to break seed dormancy. We evaluated fresh and stored seed lots using the following treatments: seed samples were planted on top of media moistened with either potassium nitrate, gibberellic acid, or distilled water as control. Two identical sets of samples were planted: one set was germinated in a chamber at alternate 15–25 °C for 28 days, while the second set was pre-chilled at 5 °C for 14 days, before moving the samples to the alternate 15–25 °C chamber for 14 days. Our findings reveal that the highest germination percentage up to 85.3 % was obtained when applying pre-chill with gibberellic acid. This novel dormancy-breaking treatment was thus effective in promoting fresh seed germination.
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Acknowledgments
The study was funded under the Strategic Scholarship Program for Frontier Research Network for the Join Ph.D. Program Thai Doctoral Degree from the Office of the Higher Education Commission, Thailand. Grateful acknowledgments are made to Khon Kaen University, Thailand Research Fund and the Office of the Higher Education Commission, Thailand for providing financial support through the Distinguished Research Professor Grant of Professor Dr. Aran Patanothai, Plant Breeding Research Center of Sustainable Agriculture of Khon Kaen University, and the Higher Education Promotion and National Research University Project of Thailand, Office of Higher Education Commission, through the Food and Functional Food Research Cluster of Khon Kaen University. Dr. Goggi’s participation in this project was partially supported by USDA-NIFA Project IOW03914. Seed Science Center, Iowa State University (ISU), Iowa, USA is acknowledged for providing laboratory facilities. Kim North is acknowledged for her practical advice and technical assistance in this trial. The North Central Regional Plant Introduction Station (NCRPIS), Ames, Iowa, USA is acknowledged for their donation of Jerusalem artichoke seeds. Dr. Laura Fredrick Marek is acknowledged for providing Jerusalem artichoke information.
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Puttha, R., Goggi, A.S., Gleason, M.L. et al. Pre-chill with gibberellic acid overcomes seed dormancy of Jerusalem artichoke. Agron. Sustain. Dev. 34, 869–878 (2014). https://doi.org/10.1007/s13593-014-0213-x
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DOI: https://doi.org/10.1007/s13593-014-0213-x