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Expression and initial characterization of a Phosphoinositide-specific phospholipase C from Populus tomentosa

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Abstract

Phosphoinositide-specific phospholipase C (PI-PLC) is a critical enzyme family, participating in various signal-transduction pathways in eukaryotic cells. However, little information on PI-PLC in plants is available. In this study, a PI-PLC gene, PtoPLC1 was obtained from Populus tomentosa and initial characterized. The recombinant PtoPLC1 protein expressed in vitro was able to hydrolyze phosphatidylinositol 4,5-biophosphate (PIP2) to generate inositol 1,4,5-trisphate (IP3) and 1,2-diacylglycerol (DAG). The catalysis of PIP2 by PtoPLC1 is Ca2+ dependent and the optimum concentration of Ca2+ is 1 μM. These results suggested that PtoPLC1 gene encodes a functional PI-PLC. The qReal-time PCR analysis revealed strong expression of PtoPLC1 in roots, but weak expression in stems and leaves. The gene is strongly induced under high salt, dehydration and ABA. These results suggest that PtoPLC1 might be involved in the signal-transduction pathways of high salt, dehydration and ABA responses in P. tomentosa.

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Abbreviations

ABA :

Abscisic acid

DAG :

1,2-diacylglycerol

IP 3 :

Inositol 1,4,5-trisphosphate

PKC :

Protein kinase C

PI-PLC :

Phosphoinositide-specific phospholipase C

PIP 2 :

Phosphatidylinositol 4,5-bisphosphate

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Acknowledgments

This work was supported by the 973 Program (No. 2012CB114501), the State 863 High-Tech Program (No. 2011AA100201), the National Natural Science Foundation of China (No. 30972392) and Beijing Academy of Agriculture and Forestry Sciences Science Foundation for Youths (No. QNJJ201416)

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Authors and Affiliations

  1. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China

    Jiewei Zhang, Zhongbao Zhang, Dan Zhu, Yang Guan, Yajuan Chen, Ruifen Li, Hongzhi Wang & Jianhua Wei

  2. National Key Laboratory of Plant Molecular Genetics, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China

    Dianyi Shi

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  1. Jiewei Zhang
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  2. Zhongbao Zhang
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Correspondence to Hongzhi Wang or Jianhua Wei.

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Zhang, J., Zhang, Z., Zhu, D. et al. Expression and initial characterization of a Phosphoinositide-specific phospholipase C from Populus tomentosa . J. Plant Biochem. Biotechnol. 24, 338–346 (2015). https://doi.org/10.1007/s13562-014-0279-1

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