Short Communication

Journal of The American Society for Mass Spectrometry

, Volume 23, Issue 6, pp 1011-1014

Open Access This content is freely available online to anyone, anywhere at any time.

How Ubiquitin Unfolds after Transfer into the Gas Phase

  • Owen S. SkinnerAffiliated withDepartment of Chemistry and Chemical Biology, Cornell University
  • , Fred W. McLaffertyAffiliated withDepartment of Chemistry and Chemical Biology, Cornell University
  • , Kathrin BreukerAffiliated withInstitute of Organic Chemistry and Center for Molecular Biosciences Innsbruck, University of Innsbruck Email author 

Abstract

The structural evolution of ubiquitin after transfer into the gas phase was studied by electron capture dissociation. Site-specific fragment yields show that ubiquitin’s solution fold is overall unstable in the gas phase, but unfolding caused by loss of solvent is slowest in regions stabilized by salt bridges.

Key words

Electron capture dissociation Protein Ubiquitin Gas phase structure