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Five Colletotrichum species are responsible for mango anthracnose in northeastern Brazil

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Abstract

Colletotrichum species are the most important and widespread form of decay affecting mango fruit worldwide. In this study, Colletotrichum species associated with fruit anthracnose isolated from mango in northeastern Brazil were subject to molecular and morphological analyses. The partial sequences of the glyceraldehyde-3-phosphate dehydrogenase gene of 143 Colletotrichum isolates was amplified, as an initial measure of genetic diversity. A subset of 47 isolates, selected to represent the range of genetic diversity and geographic origin, were further sequenced using the partial actin, β-tubulin, calmodulin, glutamine synthetase genes and rDNA-ITS region. The multilocus sequence analysis, together with a critical examination of the phenotypic characters, revealed four previously described species (Colletotrichum asianum, Colletotrichum fructicola, Colletotrichum tropicale and Colletotrichum karstii) and one new species. The new species is introduced as Colletotrichum dianesei and formally described, illustrated and compared with similar taxa. Only C. asianum and C. karstii have previously been reported from mango, while the other species represent the first report associated with the mango fruits worldwide. All species are reported for the first time associated with the mango fruits in Brazil.

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Acknowledgments

We are grateful to Sequencing Platform LABCEN/CCB in the Universidade Federal de Pernambuco for use its facilities. This work was financed by Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuc (FACEPE) and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). MPS Câmara, SJ Michereff and MA Morais Junior also acknowledge the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) research fellowship. KD Hyde thanks to NRCT of Thailand - Colletotrichum 54201020003 for support. This work was also supported by a grant from the National Plan of Science and Technology, King Abdulaziz City of Science and Technology, Riyadh, Saudi Arabia (10-Bio-965-02).

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Authors and Affiliations

  1. Departamento de Agronomia, Universidade Federal Rural de Pernambuco, 52171-900, Recife, Brazil

    Nelson B. Lima, Sami J. Michereff & Marcos P. S. Câmara

  2. Departamento de Genética, Universidade Federal de Pernambuco, 50732-970, Recife, Brazil

    Marcus Vinicius de A. Batista & Marcos A. De Morais Jr

  3. Embrapa Semiárido, 56302-970, Petrolina, Brazil

    Maria A. G. Barbosa

  4. Institute of Excellence in Fungal Research, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Kevin D. Hyde

  5. School of Science, Mae Fah Luang University, Chiang Rai, 57100, Thailand

    Kevin D. Hyde

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  1. Nelson B. Lima
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  2. Marcus Vinicius de A. Batista
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  3. Marcos A. De Morais Jr
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Correspondence to Marcos P. S. Câmara.

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Lima, N.B., de A. Batista, M.V., De Morais, M.A. et al. Five Colletotrichum species are responsible for mango anthracnose in northeastern Brazil. Fungal Diversity 61, 75–88 (2013). https://doi.org/10.1007/s13225-013-0237-6

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