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Phenotypic and genotypic diversity of root-nodulating bacteria isolated from chickpea (Cicer arietinum L.) in Iran

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Abstract

Forty-five isolates from root nodules of chickpea (Cicer arietinum L.) growing in the western and northwestern regions of Iran were characterized by phenotypic properties, repetitive element sequence-based (rep)-PCR, and 16S rRNA sequences. Based on phenotypic features, the isolates could be divided into three main groups at 92 % similarity. Nodulating isolates of those grouped in clusters II and III were grouped with reference strains of Mesorhizobium mediterraneum and M. ciceri, respectively. The three isolates of cluster I did not induce nodules on the host plant. Based on the analysis of the fingerprint patterns generated by enterobacterial repetitive intergenic consensus- and BOX-PCR, the strains were classified into six clusters (A–F) at the 75 % similarity level. These six clusters could be further divided into several subgroups at the 85 % similarity level. The phylogenetic analysis based on 16S rRNA gene sequences showed that representative isolates were closely related to M. mediterraneum, M. ciceri, and Agrobacterium tumefaciens.

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Authors and Affiliations

  1. Department of Plant Protection, Faculty of Agriculture, Buali Sina University, Hamedan, Iran

    Kiomars Rouhrazi & Gholam Khodakaramian

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  1. Kiomars Rouhrazi
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  2. Gholam Khodakaramian
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Correspondence to Gholam Khodakaramian.

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Rouhrazi, K., Khodakaramian, G. Phenotypic and genotypic diversity of root-nodulating bacteria isolated from chickpea (Cicer arietinum L.) in Iran. Ann Microbiol 65, 2219–2227 (2015). https://doi.org/10.1007/s13213-015-1062-9

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  • DOI: https://doi.org/10.1007/s13213-015-1062-9

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