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Response of cellular fatty acids to environmental stresses in endophytic Micrococcus spp.

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Abstract

The response of cellular fatty acids to various environmental stresses was studied using two endophytic species of Micrococcus. A total of 18 samples with three biological replicates from low, moderate and high stress conditions of salt (0.5, 5 and 10 % NaCl), pH (5, 7 and 10) and temperatures (15, 25 and 41 °C) were analysed. Branched chain fatty acids dominated in both the organisms, while saturated and unsaturated fatty acids were detected less frequently. The mole percentage of isoforms of branched chain fatty acids gradually increased with increasing salinity and showed more than a twofold increase at higher concentration of salt (10 %). Unlike Micrococcus yunnanensis DSM 21948T, Micrococcus aloeverae MCC 2184T showed more agreement with previous findings related to stress tolerance in other bacteria. Data indicate that iso fatty acids are responsible for the growth of Micrococcus at high salt concentration. In addition, instead of individual fatty acids, the ratio of the total content of iso/anteiso forms modulates membrane fluidity and functions during environmental stress in Micrococcus. For a comparative study of salinity stress in Gram-positive and Gram-negative bacteria, the strain of Halomonas was alsoincluded.

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Acknowledgments

This work was supported by the Department of Biotechnology (DBT; Grant no. BT/PR/0054/NDB/52/94/2007), Government of India, under the project “Establishment of microbial culture collection.”

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The authors declare that they do not have any conflict of interest regarding this manuscript.

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Authors and Affiliations

  1. Microbial Culture Collection, National Centre for Cell Science, Ganeshkhind, Pune, Maharashtra, 411007, India

    Om Prakash, Yogesh Nimonkar, Shraddha Shaligram, Neetha Joseph & Yogesh S. Shouche

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  1. Om Prakash
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  2. Yogesh Nimonkar
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  3. Shraddha Shaligram
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  4. Neetha Joseph
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  5. Yogesh S. Shouche
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Correspondence to Om Prakash.

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Prakash, O., Nimonkar, Y., Shaligram, S. et al. Response of cellular fatty acids to environmental stresses in endophytic Micrococcus spp.. Ann Microbiol 65, 2209–2218 (2015). https://doi.org/10.1007/s13213-015-1061-x

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  • DOI: https://doi.org/10.1007/s13213-015-1061-x

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