Abstract
Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strains were isolated from theGuaymas Basin hydrothermal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the strains isolated from 85°C or 95°C enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from 108°C-30MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficientmethod for isolating Pyrococcus strains. Through selecting and analyzing with multiple molecular phylogeneticmarkers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demonstrates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment.
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Foundation item: The National Basic Research Program of China under contract No. 2011CB808800; the National Natural Science Foundation of China under contract No. 40830213; the Science and Technology Commission of Shanghai Municipality under contract No. 10JC1406700; the State Key Laboratory of Ocean Engineering in China under contract No. GKZD010053-1; US-National Science Foundation under contract No. OCE-0728391.
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Liu, L., Wang, F., Xu, J. et al. Molecular diversity of Thermococcales isolated from Guaymas Basin hydrothermal vents. Acta Oceanol. Sin. 32, 75–81 (2013). https://doi.org/10.1007/s13131-013-0323-3
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DOI: https://doi.org/10.1007/s13131-013-0323-3