Skip to main content
SpringerLink
Log in

Ontogeny of in vitro shoot organogenesis from axillary meristem explants in chickpea (Cicer arietinum L.)

  • Research Article
  • Published:
Journal of Crop Science and Biotechnology Aims and scope Submit manuscript

Abstract

Chickpea (Cicer arietinum L.) is a major food legume crops for the tropics and sub-tropical regions and is the main dietary protein source for vegetarians in developing countries. Besides several abiotic factors, its production is constrained by insect pests, as well as many fungal diseases. The success of any attempt to produce resistant varieties through genetic engineering to a large extent depends on the availability of efficient and reproducible regeneration and transformation procedures. Further, the transformation techniques can be more successfully applied if the ontogeny of shoot development is well understood. Ontogeny of shoot development from axillary meristem explants (AME) in chickpea has been studied with the optimization of conditions for high-frequency multiple shoot induction. Preculture of seeds in TDZ significantly enhances the frequency of multiple shoot induction from the explants. Ontogeny and early events of multiple shoot induction revealed direct adventitious origin of the shoots. The understanding of the regeneration process could be further utilized in the designing of efficient transformation methods.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Abbreviations

BAP:

6-benzylaminopurine

IBA:

indole butyric acid

NAA:

α-Naphthalene acetic acid

PGR:

plant growth regulator

TDZ:

thidiazuron

References

  • Akcay UC, Mahmoudian M, Kamci H, Yucel M, Oktem HA. 2009. Agrobacterium tumefaciens -mediated genetic transformation of a recalcitrant grain legume, lentil (Lens culinaris Medik). Plant Cell Rep. 28: 407–417

    Article  PubMed  Google Scholar 

  • Barna KS, Wakhlu AK. 1993. Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.). Plant Cell Rep. 12: 521–524

    Article  CAS  Google Scholar 

  • Barna KS, Wakhlu AK. 1994. Whole plant regeneration of Cicer arietinum from callus cultures via organogenesis. Plant Cell Rep. 13: 510–513

    Article  CAS  Google Scholar 

  • Bean SJ, Gooding PM, Mullineaux PM, Davies DR. 1997. A simple system for pea transformation. Plant Cell Rep. 16: 513–519

    Google Scholar 

  • Brandt EB, Hess D. 1994. In vitro regeneration and propagation of chickpea (Cicer arietinum L.) from meristem tips and cotyledonary nodes. In Vitro Cell. Dev. Biol. Plant. 13: 510–513

    Google Scholar 

  • Carmen SJM, Ballester A, Vieitez AM. 2001. Effect of Thidiazuron on multiple shoot induction and plant regeneration from cotyledonary nodes of chestnut. J. Hort. Sci. Biotechnol. 76: 588–595

    Google Scholar 

  • Chakraborti D, Sarkar A, Das D. 2006. Efficient and rapid in vitro plant regeneration system for Indian cultivars of chickpea (Cicer arietinum L.). Plant Cell Tissue Organ Cult. 86: 117–123

    Article  Google Scholar 

  • Fortes AM, País MS. 2000. Organogenesis from internode-derived nodules of Humulus lupulus L. var Nugget (Cannabinaceae): Histological studies and changes in the starch content. Am. J. Bot. 87: 971–979

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K. 1968. Nutrient requirements for suspension cultures of soybean root cells. Exp. Cell Res. 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Hinchee MAW, Connor-Ward D, Christine AN, McDonald RE, Sato SJ, Gasser CS, Fischoff DA, Re DB, Fraley RT, Horsch RB. 1988. Production of transgenic soybean plants using Agrobacterium-mediated DNA transfer. Nat. Biotech 6: 912–922

    Google Scholar 

  • Jayanand B, Sudarsanam G, Sharma KK. 2003. An efficient protocol for the regeneration of whole plants of chickpea (Cicer arietinum L.) by using axillary meristem explants derived from in vitro germinated seedlings. In Vitro Cell. Dev. Biol. Plant. 39: 171–179

    Article  Google Scholar 

  • Kar S, Johnson TM, Nayak P, Sen SK. 1996. Efficient transgenic plant regeneration through Agrobacterium mediated transformation of chickpea (Cicer arietinum L.). Plant Cell Rep. 16: 32–37

    Article  CAS  Google Scholar 

  • Kiran G, Kaviraj, CP, Jogeswar, G, Kishor KVK, Rao S. 2005. Direct and high frequency somatic embryogenesis and plant regeneration from hypocotyls of chickpea (Cicer arietinum L.), a grain legume. Curr. Sci. 89: 1012–1018

    Google Scholar 

  • Krishnamurthy KV, Suhasini K, Sagree AP, Meixner M, de Kathen A, Pickardt T, Schieder O. 2000. Agrobacterium mediated transformation of chickpea (Cicer arietinum L.) embryo axes. Plant Cell Rep. 19: 235–240

    Article  CAS  Google Scholar 

  • Kumar VD, Kirti PB, Sachan JKS, Chopra VL. 1994. Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.). Plant Cell Rep. 13: 468–472

    Article  CAS  Google Scholar 

  • Malik KA, Saxena PK. 1992. Regeneration in (Phaseolus vulgaris L.) high frequency induction of direct shoot formation in intact seedlings by N6-Benzylaminopurine and thidiazuron. Planta 186: 384–389

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F. 1962. A revised method for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Article  CAS  Google Scholar 

  • Onamu R, Obukosia SD, Musembi N, Hutchinson MJ. 2003. Efficacy of thidiazuron in in vitro propagation of carnation shoot tips: Influence of dose and duration of exposure. Afr. Crop Sci. J. 11: 125–132

    Google Scholar 

  • Polanco MC, Ruiz, ML. 1997. Effect of benzylaminopurine on in vitro and in vivo root development in Lentil, Lens culinaris Medik. Plant Cell Rep. 17: 22–26

    Article  Google Scholar 

  • Polisetty R, Paul V, Deveshwar JJ, Khetarpal S, Suresh K, Chandra R. 1997. Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.). Plant Cell Rep. 16: 565–571

    CAS  Google Scholar 

  • Prakash S, Chowdhary JB, Jain RK, Chowdhary VK. 1992. Factors affecting plant regeneration in chickpea, Cicer arietinum L. Ind. J. Exp. Biol. 30: 1149–1153

    Google Scholar 

  • Sagare AP, Suhasini K, Krishnamurthy KV. 1993. Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.). Plant Cell Rep. 12: 652–655

    Article  Google Scholar 

  • Sharma HC, Sharma KK, Seetharama N, Oritz R. 2001. Genetic transformation of crop plants: Risk and opportunities for the rural poor. Curr. Sci. 80: 1495–1508

    CAS  Google Scholar 

  • Sharma VK, Hansch R, Mendel RR, Schulze J. 2005. Influence of picloram and thidiazuron on high frequency plant regeneration in elite cultivars of wheat with long-term retention of morphogenecity using meristemic shoot segments. Plant Breed. 124: 242–246

    Article  CAS  Google Scholar 

  • Singh R, Jat RS, Sahoo PD, Srinivasan. 2002. Thidiazuron induced multiple shoot formation in chickpea, Cicer arietinum L. J. Plant Biochem. Biotechnol. 11: 129–131

    CAS  Google Scholar 

  • Subhadra, Vashisht RK, Chowdhury JB, Singh M, Sareen PK. 1998. Multiple shoots from cotyledonary node explants of non-nodulating genotype (ICC435M) of chickpea, Cicer arietinum L. Ind. J. Exp. Biol. 36: 1276–1279

    Google Scholar 

  • Wright MS, Koehler SM, Hinchee MA, Carmes MG. 1986. Plant regeneration by organogenesis in Glycine max. Plant Cell Rep. 5: 150–154

    Article  CAS  Google Scholar 

  • Yoshida T. 2002. Adventitious shoot formation from hypocotyls sections of mature soybean seeds. Breed. Sci. 52: 1–8

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Crop Improvement Division, Indian Institute of Pulses Research, Kanpur, 208024, India

    Jaya Srivastava, Alok Das, Khela Ram Soren, Sushil Kumar Chaturvedi, Nagaswamy Nadarajan & Subhojit Datta

Authors
  1. Jaya Srivastava
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Alok Das
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Khela Ram Soren
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Sushil Kumar Chaturvedi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Nagaswamy Nadarajan
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Subhojit Datta
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Subhojit Datta.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Srivastava, J., Das, A., Soren, K.R. et al. Ontogeny of in vitro shoot organogenesis from axillary meristem explants in chickpea (Cicer arietinum L.). J. Crop Sci. Biotechnol. 15, 245–250 (2012). https://doi.org/10.1007/s12892-012-0032-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12892-012-0032-z

Key words

Search

Navigation