Abstract
Hypancistrus zebra is an ornamental fish endemic to the Xingu river, Amazon basin, which is under the impact zone of the world’s fourth-largest hydroelectric dam. Illegal capture is another threat to this species. Despite its critical conservation status only two nucleotide sequences from this fish are publicly available on GenBank and on the BOLD System. Here, the nearly complete mitochondrial genome of H. zebra is described; totalizing 16,330 nucleotides, including the complete sequences of the two ribosomal RNA subunits, the 22 transfer RNAs and the 13 protein coding genes. The mitochondrial genome was assembled from transcriptome data, from seven organs, two individual fish, and to an average sequencing depth of 7245x. Seven nucleotide differences were found between the individual fish sequenced here-in, while four were detected among these individuals and the unique mitochondrial sequence publicly available. Additionally, 21 heteroplasmic sites were found among seven organs. This is the first quasi complete mitochondrial genome of a species belonging to the Peckoltia Clade, a Loricariidae phylogenetically problematic tribe. This genetic resource will be valuable in the efforts to elucidate the phylogenetic relationships among the Peckoltia Clade species, as well as it shall subsidize future practices aiming the conservation of this endangered species.
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Acknowledgements
Authors are grateful to Dr. Jansen Zuanon from the Instituto Nacional de Pesquisas da Amazônia (INPA) for the donation of Hypancistrus zebra specimens (Voucher INPA 46655) used in this study. T.E.P. thanks Dr. Adalberto L. Val, Nazaré Paula and the entire ADAPTA and LEEM staffs for their hospitality and assistance during his visit to INPA. This work was supported by the U.S. Agency for International Development under Grants PGA-2000003446 and PGA-2000004790; and by the Coordenação de Aperfeiçoamento de Pessoal de Nivel Superior (CAPES) from the Brazilian Government for a master fellowship for M. G. P. M., a doctoral fellowship for D. A. M., and a postdoctoral fellowship to T. E. P.
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Magalhães, M.G.P., Moreira, D.A., Furtado, C. et al. The mitochondrial genome of Hypancistrus zebra (Isbrücker & Nijssen, 1991) (Siluriformes: Loricariidae), an endangered ornamental fish from the Brazilian Amazon. Conservation Genet Resour 9, 319–324 (2017). https://doi.org/10.1007/s12686-016-0645-5
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DOI: https://doi.org/10.1007/s12686-016-0645-5