Abstract
A comprehensive study was conducted on camel ticks to assess the epidemiological aspects of the infection in camels. From May 2012 to January 2013, 11 cities and towns from the Khorasan provinces, northeastern Iran, were randomly selected as a “cluster” and at least 14 camels were sampled from each cluster. A total of 200 camels were examined in this study, reverse transcriptase polymerase chain reaction was used for the detection of the Crimean–Congo hemorrhagic fever virus (CCHFV) genome. Tick infestation was observed in 171 of the 200 camels, 480 ixodid ticks were collected, and one genus was identified as Hyalomma. Four species were reported to be the major tick species infesting camels. Among these, Hyalomma dromedarii was the most predominant tick species (90.7 %), followed by H. anatolicum (6 %), H. marginatum (2.9 %), and H. asiaticum (0.4 %). The genome of the CCHFV was detected in 49 (10.2 %) of the 480 ticks. The CCHFV RNA was detected in two of the four tick species, and the viral genome was detected from tick samples in three South Khorasan cities. The positivity rate of ticks was as follows: Boshroyeh, 25 out of 480 (5.2 %); Birjand, 17 out of 480 (3.5 %); and Nehbandan, 7 out of 480 (1.5 %). We recommend the use of acaricides to prevent disease transmission to humans and to reduce the tick population in camels. Care should be taken by abattoir workers and by those who work closely with camels.
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References
Apanaskevich DA, Filippova NA (2007) Larval identification of species and subspecies of the genus Hyalomma (Acari: Ixodidae) from Russia and neighboring territories. Parazitolojiia 41:268–283
Athar MN, Baqai HZ, Ahmad M, Khalid MA, Bashir N, Ahmad AM, Balouch AH, Bashir K (2003) Crimean–Congo hemorrhagic fever outbreak in Rawalpindi, Pakistan. Am J Trop Med Hyg 69:284–287
Bishop DH (1996) Biology and molecular biology of bunyaviruses. In: Elliott RM (ed) The bunyaviridae. Plenum Press, New York, pp 19–61
Chinikar S, Persson SM, Johansson M, Bladh L, Goya M, Houshmand B, Mirazimi A, Plyusnin A, Lundkvist A, Nilsson M (2004) Genetic analysis of Crimean–Congo hemorrhagic fever virus in Iran. J Med Virol 73(3):404–411
Chinikar S, Ghiasi SM, Hewson R, Moradi M, Haeri A (2010) Crimean–Congo hemorrhagic fever in Iran and neighboring countries. J Clin Virol 47:110–114
Chinikar S, Shayesteh M, Khakifirouz S, Jalali T, Rasi Varaie FS, Rafigh M, Mostafavi E, Shah-Hosseini N (2012a) Nosocomial infection of Crimean–Congo hemorrhagic fever in eastern Iran: case report. TMAID 11(4):252–255
Chinikar S, Shah-Hosseini N, Bouzari S, Jalali T, Shokrgozar MA, Mostafavi E (2012b) New circulating genomic variant of Crimean–Congo hemorrhagic fever virus in Iran. Arch Virol 158(5):1085–1088
Drosten C, Minnak D, Emmerich P, Schmitz H, Reinicke T (2002) Crimean–Congo hemorrhagic fever in Kosovo. J Clin Microbiol 40:1122–1123
Duh D, Nichol ST, Khristova ML, Saksida A, Hafner Bratkovic I, Petrovec M, Dedushaj I, Ahmeti S, Avsic-Zupanc T (2008) The complete genome sequence of a Crimean–Congo hemorrhagic fever virus isolated from an endemic region in Kosovo. Virol J 5:7. doi:10.1186/1743-422X-5-7
Durden LA, Logan TM, Wilson ML, Linthicum KJ (1993) Experimental vector incompetence of a soft tick, Ornithodoros sonrai (Acari: Argasidae), for Crimean–Congo hemorrhagic fever virus. J Med Entomol 30(2):493–496
Maha A, El tigani, Mohammed AS (2010) Ticks infesting camels in El Butana area Mid-Central Sudan. Sudan J Vet Res 25:51–54
Ergonul O (2006) Crimean–Congo hemorrhagic fever. Lancet Infect Dis 6:203–214
Estrada-Pena A, Farkas R, Jaenson T, Koenen F, Madder M, Pascucci I, Salman M, Tarrés-Call J, Jongejan F (2013) Association of environmental traits with the geographic ranges of ticks (Acari: Ixodidae) of medical and veterinary importance in the western Palearctic. Exp Appl Acarol 59:351–366
Hoogstraal H (1956) African ixodidae, tick of the sudan with special reference to Equatoria province and with preliminary reviews of the genera boophilus, Margaropus and Hyalomma. Washington DC US, pp.1101-103
Hoogstraal H (1979) The epidemiology of tick-borne Crimean–Congo hemorrhagic fever in Asia, Europe, and Africa. J Med Entomol 15:307–317
Kaiser MN, Hoogstraal H (1963) The hyalomma ticks (Ixodide) of Afghanistan. J Parasitol 49:130–139
Lawal MD, Amen IG, Ahmed A (2007) Some ectoparasites of Camelus dromedarius in Soko Nigeria. J Entomol 4:143–148
Mehravaran A, Moradi M, Telmadarraiy Z, Mostafavi E, Moradi AR, Khakifirouz S, Shah-Hosseini N, Rasi Varaie FS, Jalali T, Hekmat S, Ghiasi SM, Chinikar S (2013) Molecular detection of Crimean–Congo hemorrhagic fever (CCHF) virus in ticks from southeastern Iran. Ticks Tick Borne Dis 4:35–38
Nabian S, Rahbari S, shayan P, Haddadzadeh HR (2007) Occurrence of soft and hard ticks on ruminants in Zagros Mountainous Areas of Iran. Iran J Parasito l2:12-17
Nazifi S, Tamadon A, Behzadi MA, Hadadi S, Raayat Jahromi AR (2011) One-humped camels (Camelus dromedaries) hard ticks infestation in Qeshm Island, Iran. Vet Res Forum 2:135–138
Rahbari S, Nabian S, Shayan P (2007) Primary report on distribution of tick fauna in Iran. Parasitol Res 101(Suppl 2):175–177
Saijo M, Qing T, Niikura M, Maeda A, Ikegami T, Sakai K, Prehaud C, Kurane I, Morikawa S (2002) Immunofluorescence technique using HeLa cells expressing recombinant nucleoprotein for detection of immunoglobulin G antibodies to Crimean–Congo hemorrhagic fever virus. J Clin Microbiol 40(2):372–375
Salim Abadi Y, Chinikar S, Telmadarriy Z, Vatandoost H, Moradi M, Oshaghi AM, Ghiasi M (2011) Crimean–Congo hemorrhagic fever: a molecular survey on hard ticks (Ixodidae) in Yazd province, Iran. Asian Pac J Trop Med 4(1):61–63
Salimabadi Y, Telmadarriy Z, Vatandoost H, Chinikar S, Oshaghi MA, Moradi M, Mirabzadeh V, Ardakan E, Hekmat S, Nasiri A (2010) Hard ticks on domestic ruminants and their seasonal population dynamics in Yazd province Iran. Iran J Arthropod Borne Dis 4:66–71
Swanepoel R, Shepherd AL, Leman PA, Shepherd SP, Mc Gillivray GM, Erasmus MJ, Searle LA, Gill DE (1987) Epidemiologic and clinical features of Crimean–Congo hemorrhagic fever in southern Africa. Am J Trop Med Hyg 36(1):120–132
Tahmasebi F, Ghiasi SM, Mostafavi E, Moradi M, Piazak N, Mozafari A, Haeri A, Fooks AR, Chinikar S (2010) Molecular epidemiology of Crimean–Congo hemorrhagic fever virus genome isolated from ticks of Hamadan province of Iran. J Vector Borne Dis 47(4):211–216
Telmadarraiy Z, Ghiasi SM, Moradi M, Vatandoost H, Eshraghian MR, Faghihi F, Zarei Z, Haeri A, Chinikar S (2010) A survey of Crimean–Congo hemorrhagic fever in livestock and ticks in Ardabil Province, Iran during 2004–2005. Scand J Infect Dis 42(2):137–141
Whitehouse CA (2004) Crimean–Congo hemorrhagic fever. Antiviral Res 64(3):145–160
Acknowledgments
The project was funded by Arboviruses and Viral Hemorrhagic Fever Laboratory, National Reference Lab, Pasteur Institute Tehran-Iran and Ferdowsi University of Mashhad, bearing registration code 3/230667. Thanks to staff members of veterinary department of the North Khorasan province, particularly Dr Razavi, Dr Ramezani, Dr Ahmadi, Dr Hassan Safaei and Dr Shahin Ahmadi for their collaboration in sampling. The authors really appreciate Dr Hussein Janati, Nariman Shah-Hosseini, Sahar Khakifirouz, and Tahmineh Jalali for sincerely technical support.
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Champour, M., Chinikar, S., Mohammadi, G. et al. Molecular epidemiology of Crimean–Congo hemorrhagic fever virus detected from ticks of one humped camels (Camelus dromedarius) population in northeastern Iran. J Parasit Dis 40, 110–115 (2016). https://doi.org/10.1007/s12639-014-0458-y
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DOI: https://doi.org/10.1007/s12639-014-0458-y