Abstract
This study was performed to determine the viability of Lactobacillus acidophilus and Bifidobacterium bifidum in yogurt made with strawberry marmalade (SM) and to examine the quality properties of probiotic yogurt. Acidity, pH, bacterial counts and sensory analysis of the yogurt samples were investigated on days 1, 3, 5, 7, 10 and 14 during storage at 4 °C. The survival rate of L. acidophilus was greater than that of B. bifidum. The viability of L. acidophilus decreased during the storage period, but B. bifidum numbers remained stable during the storage period. The highest L. acidophilus count (7.20 log cfu/g) was found in L. acidophilus + B. bifidum SM yogurt on day 1. The highest B. bifidum count (6.13 log cfu/g) was detected in yogurt containing L. acidophilus + B. bifidum SM yogurt on day 7. Yeast and mould counts of all yogurts increased during the storage period. Coliform bacteria and Staphylococcus aureus were not detected in the yogurt samples. The highest overall acceptance sensory score was observed in yogurts containing L. acidophilus. Considering the sensory and probiotic characteristics of all yogurt samples, this study suggested that strawberry yogurt with a suitable 5–7 day storage period can be produced with single L. acidophilus addition or single B. bifidum addition.
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References
Cruz AG, Castro WF, Faria JAF, Bolini HMA, Celeghini RMS, Raices RSL, Oliveira CAF, Freitas MQ, Conte Junior CA, Marsico ET (2013) Stability of probiotic yogurt added with glucose oxidase in plastic materials with different permeability oxygen rates during the refrigerated storage. Food Res Int 51:723–728
Batista ALD, Silva R, Cappato LP, Almada CN, Garcia RKA, Silva MC, Raices RSL, Arellano DB, Sant'Ana AS, Conte Junior CA, Freitas MQ, Cruz AG (2015) Quality parameters of probiotic yogurt added to glucose oxidase compared to commercial products through microbiological, physical–chemical and metabolic activity analyses. Food Res Int 77:627–635
Parvez S, Malik KA, Ah Kang S, Kim HY (2006) Probiotics and their fermented food products are beneficial for health. J Appl Microbiol 100:1171–1185
Turgut T, Çakmakçı S (2009) Investigation of the possible use of probiotics in ice cream manufacture. Int J Dairy Technol 62:444–451
Hosono A, Kitazawa H, Yamaguchi T (1997) Antimutagenic and antitumour activities of lactic acid bacteria. R. Fuller (ed.), In: Probiotics 2. (pp. 89–132), Springer Netherlands
Fooks LJ, Fuller R, Gibson GR (1999) Prebiotics, probiotics and human gut microbiology. Int Dairy J 9:53–61
Avonts L, Uytven EV, de Vuyst L (2004) Cell growth and bacteriocin production of probiotic Lactobacillus strains in different media. Int Dairy J 14(11):947–955
Heenan CN, Adams MC, Hosken RW, Fleet GH (2004) Survival and sensory acceptability of probiotic microorganisms in a nonfermented frozen vegetarian dessert. LWT-Food Sci Technol 37:461–466
Stanton C, Ross RP, Fitzgerald GF, Van Sinderen D (2005) Fermented functional foods based on probiotics and their biogenic metabolites. Curr Opin Biotechnol 16:198–203
Vinderola CG, Bailo N, Reinheimer JA (2000) Survival of probiotic microflora in Argentinian yoghurts during refrigerated storage. Food Res Int 33:97–102
Lourens-Hattingh A, Viljoen BC (2001) Yoghurt as probiotic carrier food. Int Dairy J 11:1–17
Kailasapathy K, Harmstorf I, Phillips M (2008) Survival of Lactobacillus acidophilus and Bifidobacterium animalis ssp. lactis in stirred fruit yogurts. LWT-Food Sci Technol 41:1317–1322
Nagpal R, Kumar A, Kumar M, Behare PV, Jain S, Yadav H (2012) Probiotics, their health benefits and applications for developing healthier foods: a review. FEMS Microbiol Lett 334:1–15
Del Campo R, Bravo D, Canton R, Ruiz-Garbajosa P, Garcia-Albiach R, Montesi-Libois A, Yuste FJ, Abraira V, Baquero F (2005) Scarce evidence of yogurt lactic acid bacteria in human feces after daily yogurt consumption by healthy volunteers. Appl Environ Microbiol 71:547–549
Ashraf R, Shah NP (2011) Selective and differential enumerations of Lactobacillus delbrueckii subsp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Lactobacillus casei and Bifidobacterium spp. in yoghurt—a review. Int J Food Microbiol 149:194–208
Guarner F, Perdigon G, Corthier G, Salminen S, Koletzko B, Morelli L (2005) Should yoghurt cultures be considered probiotic? Brit J Nutr 93:783–786
Mater DDG, Bretigny L, Firmesse O, Flores MJ, Mogenet A, Bresson JL, Corthier G (2005) Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus survive gastrointestinal transit of healthy volunteers consuming yogurt. FEMS Microbiol Lett 250:185–187
Çakmakçı S, Çetin B, Turgut T, Gürses M, Erdoğan A (2012) Probiotic properties, sensory qualities, and storage stability of probiotic banana yogurts. Turk J Vet Anim Sci 36:231–237
Gomes AMP, Malcata FX (1999) Bifidobacterium spp. and Lactobacillus acidophilus: biochemical, technological and therapeutical properties relevant for use as probiotics. Trends Food Sci Technol 10(4/5):139–157
Alamprese C, Foschino R, Rossi M, Pompei C, Savani L (2002) Survival of Lactobacillus johnsonii La1 and influence of its addition in retail-manufactured ice cream produced with different sugar and fat concentrations. Int Dairy J 12:201–208
Saarela M, Lähteenmäki L, Crittenden R, Salminen S, Mattila Sandholm T (2002) Gut bacteria and health foods—the European perspective. Int J Food Microbiol 78:99–117
Hoesl CE, Altwein JE (2005) The probiotic approach: an alternative treatment option in urology. Eur Urol 47:288–296
Penner R, Fedorak RN, Madsen KL (2005) Probiotics and nutraceuticals: non-medicinal treatments of gastrointestinal diseases. Curr Opin Pharmacol 5(6):596–603
Cruz AG, Cadena RS, Faria JAF, Oliveira CAF, Cavalcanti RN, Bona E, Bolini HMA, Da Silva MAAP (2011) Consumer acceptability and purchase intent of probiotic yoghurt with added glucose oxidase using sensometrics, artificial neural networks and logistic regression. Int J Dairy Technol 64:549–556
Ertem H, Çakmakçı S (2016) The determination of shelf life and some quality characteristics of yoghurts produced with the addition of probiotic bacteria and Gobdin. International Congress on Food of Animal Origin (Abstract Book, 189–190). 10–13 November, North Cyprus.
Vinderola CG, Reinheimer JA (1999) Culture media for the enumeration of Bifidobacterium bifidum and Lactobacillus acidophilus in the presence of yoghurt bacteria. Int Dairy J 8:497–505
Kneifel W, Pacher B (1993) An X-Glu based agar medium for the selective enumeration of Lactobacillus acidophilus in yogurt-related milk products. Int Dairy J 3(3):277–291
Dave RI, Shah NP (1996) Evaluation of media for selective enumeration of Streptococcus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus, Lactobacillus acidophilus, and bifidobacteria. J Dairy Sci 79(9):1529–1536
Fluka (2013) Fluka microbiology manual 4th edn. Fluka GmbH, Sigma-Aldrich Co. LLC. St. Louis, USA.
Harrigan WF (1998) Laboratory methods in food microbiology, 3rd edn. Academic Press, London
Kurt A, Çakmakçı S, Çağlar A (1996) A guidebook of analysis methods of milk and milk products. pp 238. Erzurum, Turkey: Faculty of Agriculture, Atatürk University.
Gürsel A, Karacabey A (1998) Dondurma Teknolojisine İlişkin Hesaplamalar, Reçeteler ve Kalite Kontrol Testleri. Ankara Üniv. Ziraat Fak. Yay. No: 1498, Ankara, Turkey.
Roland AM, Phillips LG, Boor KJ (1999) Effects of fat content on the sensory properties, melting, color and hardness of ice cream. J Dairy Sci 82(1):32–38
Chung SJ, Heymann H, Grün IU (2003) Application of GPA and PLSR in correlating sensory and chemical data sets. Food Qual Prefer 14:485–495
Arslan S, Bayrakci S (2016) Physicochemical, functional, and sensory properties of yogurts containing persimmon. Turk J Agric For 40(1):68–74
Kumar A, Kumar D (2016) Development of antioxidant rich fruit supplemented probiotic yogurts using free and microencapsulated Lactobacillus rhamnosus culture. J Food Sci Technol 53:667–675
Cruz AG, Walter EHM, Cadena RS, Faria JAF, Bolini MAH, Pinheiro HP, Sant’Ana AS (2010) Survival analysis methodology to predict the shelf-life of probiotic flavored yogurt. Food Res Int 43:1444–1448
Bakırcı I, Kavaz A (2008) An investigation of some properties of banana yogurts made with commercial ABT-2 starter culture during storage. Int J Dairy Technol 61(3):270–276
Öztürk BA, Öner MD (1999) Production and evaluation of yogurt with concentrated grape juice. J Food Sci 64:530–532
Çon AH, Çakmakçı S, Çağlar A, Gökalp HY (1996) Effects of different fruits and storage periods on microbiological qualities of fruit-flavored yogurt produced in Turkey. J Food Prot 59:402–406
Mataragas M, Dimitriou V, Skandamis PN, Drosinos EH (2011) Quantifying the spoilage and shelf life of yogurt with fruits. Food Microbiol 28:611–616
Heller KJ (2001) Probiotic bacteria in fermented foods: product characteristics and starter organisms. Am J Clin Nutr 73(suppl):374S–379S
Akalın AS, Fenderya S, Akbulut N (2004) Viability and activity of bifidobacteria in yoghurt containing fructooligosaccharide during refrigerated storage. Int J Food Sci Technol 39(6):613–621
Ranadheera CS, Evans CA, Adams MC, Baines SK (2012) Probiotic viability and physico-chemical and sensory properties of plain and stirred fruit yogurts made from goat’s milk. Food Chem 135(3):1411–1418
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We would like to thank the Atatürk University Research Fund for supporting this research.
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Turgut, T., Cakmakci, S. Probiotic Strawberry Yogurts: Microbiological, Chemical and Sensory Properties. Probiotics & Antimicro. Prot. 10, 64–70 (2018). https://doi.org/10.1007/s12602-017-9278-6
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DOI: https://doi.org/10.1007/s12602-017-9278-6