Article

Phytoparasitica

, Volume 42, Issue 3, pp 381-386

Present status of Citrus tristeza virus infecting Citrus spp. in Darjeeling hills and its detection in different plant parts

  • Amalendu GhoshAffiliated withIndian Agricultural Research Institute, Regional Station
  • , Amrita DasAffiliated withIndian Agricultural Research Institute, Regional Station Email author 
  • , K. B. PunAffiliated withIndian Agricultural Research Institute, Regional StationRegional Rainfed Lowland Rice Research Station, Central Rice Research Institute
  • , R. KumarAffiliated withIndian Agricultural Research Institute, Regional StationIndian Agricultural Research Institute, Regional Station
  • , Ramu MeenaAffiliated withIndian Agricultural Research Institute, Regional Station
  • , V. K. BaranwalAffiliated withAdvanced Centre for Plant Virology, Indian Agricultural Research Institute

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Abstract

Darjeeling hills of West Bengal (India) are well known for the production of mandarin orange (Citrus reticulata). The spread of Citrus tristeza virus (CTV) in this area has threatened the cultivation of citrus. Therefore, a study of the incidence and distribution of CTV in Darjeeling hills was undertaken and a geographical map was prepared. In DAS-ELISA, a high titre of CTV was recorded in the tender shoot bark, leaf petiole and mid-rib of seven Citrus spp. Three citrus species, viz., pomelo, trifoliate and kumquat, were found to be free of CTV infection when tested in double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcription polymerase chain reaction (RT-PCR).

Keywords

Citrus reticulata Distribution India Mandarin orange Tristeza CTV Darjeeling