Present status of Citrus tristeza virus infecting Citrus spp. in Darjeeling hills and its detection in different plant parts
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Darjeeling hills of West Bengal (India) are well known for the production of mandarin orange (Citrus reticulata). The spread of Citrus tristeza virus (CTV) in this area has threatened the cultivation of citrus. Therefore, a study of the incidence and distribution of CTV in Darjeeling hills was undertaken and a geographical map was prepared. In DAS-ELISA, a high titre of CTV was recorded in the tender shoot bark, leaf petiole and mid-rib of seven Citrus spp. Three citrus species, viz., pomelo, trifoliate and kumquat, were found to be free of CTV infection when tested in double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA) and reverse transcription polymerase chain reaction (RT-PCR).
- Ahlawat, Y. S., Chakraborty, N. K., Verma, A., & Raychoudhury, S. P. (1992). Diagnosis of diseases caused by viruses and fastidious prokaryotes in citrus in India. Proceedings of the International Society of Citriculture, 2, 749–751.
- Ayazpour, K., Sijam, K., Vadamalai, G., & Jaafar, H. (2011). Status of citrus tristeza virus (CTV) in Peninsular Malaysia. African Journal of Microbiology Research, 5, 838–843.
- Bar-Joseph, M., & Dawson, W. O. (2008). Citrus tristeza virus. Encyclopedia of Virology, 1, 520–525. CrossRef
- Bar-Joseph, M., Marcus, R., & Lee, R. F. (1989). The continuous challenge of citrus tristeza virus control. Annual Review of Phytopathology, 27, 291–316. CrossRef
- Biswas, K. K. (2008). Molecular diagnosis of citrus tristeza virus in mandarin orchards of Darjeeling hills of West Bengal. Indian Journal of Virology, 19, 26–31.
- Biswas, K. K., Manjunath, K. L., Lee, R. F., & Ahlawat, Y. S. (2004). Identification of diversified Citrus tristeza virus population isolates from eastern Himalayan region of India by heteroduplex mobility assay and nucleotide sequence analysis. In: National Symposium on Molecular Diagnostics for the Management of Viral Diseases (New Delhi, India).
- Biswas, K. K., Tarafdar, A., & Sharma, S. K. (2012). Complete genome sequence of mandarin decline Citrus tristeza virus of the Northeastern Himalayan hill region of India: comparative analyses determine recombinant. Archives of Virology, 157, 579–583. CrossRef
- Brlansky, R. H., Lee, R. F., & Garnsey, G. M. (1988). In situ immunofluorescence for the detection of Citrus tristeza virus inclusion bodies. Plant Disease, 72, 1039–1041. CrossRef
- Clark, M. F., & Adams, A. N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34, 475–483. CrossRef
- Fagoaga, C., López, C., Moreno, P., Navarro, L., Flores, R., & Peña, L. (2005). Viral-like symptoms induced by the ectopic expression of the p23 gene of Citrus tristeza virus are citrus specific and do not correlate with the pathogenicity of the virus strain. Molecular Plant-Microbe Interactions, 18, 435–445. CrossRef
- Karasev, A. V., Boyko, V. P., Gowda, S., Nikolaeva, O. V., Hilf, M. E., Koonin, E. V., et al. (1995). Complete sequence of the citrus tristeza virus RNA genome. Virology, 208, 511–520. CrossRef
- Mukhopadhyay, S., Mukhopadhyay, K., Mukherjee, N., Ghosh, M. R., Bose, T. K., Choudhury, A. K., et al. (1986). The state of mandarin orange cultivation in Darjeeling District. S. P. Roychoudhury, & J. P. Verma, (Eds.) Review of Tropical Plant Pathology, 2, 35–53.
- Ruiz-Ruiz, S., Morenoa, P., Guerri, J., & Ambros, S. (2007). A real-time RT-PCR assay for detection and absolute quantitation of citrus tristeza virus in different plant tissues. Journal of Virological Methods, 145, 96–105. CrossRef
- Present status of Citrus tristeza virus infecting Citrus spp. in Darjeeling hills and its detection in different plant parts
Volume 42, Issue 3 , pp 381-386
- Cover Date
- Print ISSN
- Online ISSN
- Springer Netherlands
- Additional Links
- Citrus reticulata
- Mandarin orange
- Industry Sectors
- Author Affiliations
- 1. Indian Agricultural Research Institute, Regional Station, Kalimpong, 734 301, West Bengal, India
- 3. Regional Rainfed Lowland Rice Research Station, Central Rice Research Institute, Gerua, 781 102, Assam, India
- 4. Indian Agricultural Research Institute, Regional Station, Karnal, 132 001, Haryana, India
- 2. Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110 012, India