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Complete genome sequence of mandarin decline Citrus tristeza virus of the Northeastern Himalayan hill region of India: comparative analyses determine recombinant

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Abstract

The complete genome sequence of a mandarin (Citrus reticulata) decline CTV isolate, Kpg3, of the Darjeeling hills of the Northeastern Himalayan region of India is reported for the first time. The complete Kpg3 genome has 19253 nt, and its nucleotide sequence identity ranged from 79% with the Florida CTV isolate T36 to 94% with the Israel isolate VT, whereas its identity to B165, the other Indian isolate, was 89%. Phylogenetic analysis indicated that the Kpg3 genome is closely related to isolate VT and distantly to T36 and B165. Recombination analysis indicated that Kpg3 is recombinant and originated through multiple recombination events in which parts of the genome were exchanged between divergent CTV sequences.

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Acknowledgments

The authors are grateful to Richard Lee and K.L. Manjunath, USDA-ARS, NCGRCD, Riverside, for teaching HMA analysis and for their valuable suggestions. This work was supported by Research Project (Code No. 24-33), Department of Biotechnology, Govt. of India. We are also thankful to Dr. R. K. Jain, Head, Division of Plant Pathology; Dr. V. G. Malathi, Ex-In-charge, ACPV; Director, Indian Agricultural Research Institute (IARI), New Delhi for providing financial and laboratory support.

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  1. Division of Plant Pathology, Advanced Centre for Plant Virology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Kajal K. Biswas, Avijit Tarafdar & Susheel K. Sharma

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  1. Kajal K. Biswas
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  2. Avijit Tarafdar
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  3. Susheel K. Sharma
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Correspondence to Kajal K. Biswas.

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Biswas, K.K., Tarafdar, A. & Sharma, S.K. Complete genome sequence of mandarin decline Citrus tristeza virus of the Northeastern Himalayan hill region of India: comparative analyses determine recombinant. Arch Virol 157, 579–583 (2012). https://doi.org/10.1007/s00705-011-1165-y

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