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Leptin receptor overlapping transcript: characterization, tissue distribution and changes in gene expression in response to eyestalk ablation in the Pacific white shrimp Litopenaeus vannamei

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Abstract

The leptin receptor overlapping transcript (LEPROT), which arose evolutionarily before the leptin receptor, is involved in the nutritional regulation of metabolism and reproduction. Eyestalk ablation is performed on brood stock to induce rapid gonad development in the Pacific white shrimp Litopenaeus vannamei. To clarify the role of L. vannamei leprot (Lvleprot) in gonadal development, Lvleprot cDNA was isolated and a phylogenetic tree was constructed based on the LEPROT amino acid sequences of shrimp, other invertebrates and vertebrates. The resulting topology clearly reflected the taxonomic relationships of the selected organisms. Alignment of LEPROT amino acid sequences showed that there was very high similarity between LvLEPROT and LEPROT from other invertebrates. Quantitative polymerase chain reaction showed that Lvleprot expression was highest in the gonads and intestine, moderate in the hepatopancreas, androgenic gland and muscle, and lowest in gill, heart and stomach, without a sexually dimorphic pattern difference between males and females. After eyestalk ablation, the gonadosomatic index increased significantly, but the hepatosomatic index decreased significantly. In addition, Lvleprot transcript levels in the gonads and intestine decreased significantly, but were significantly increased in the hepatopancreas after eyestalk ablation. These results indicate the significant roles of Lvleprot in the nutritional regulation of reproduction during the period of rapid development in Pacific white shrimp.

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Acknowledgments

This work was supported by grants from the Project of Guangdong Ocean and Fisheries Science & Natural Science (A201608B01), the Natural Science Foundation of Guangdong Province (2016A030313743), the Project of Provincial Key Platform and Major Scientific Research of Colleges and Universities in Guangdong (2015KTSCX058), the Sail Project of Guangdong Province (2014 no. 1). the Project of Enhancing School with Innovation of Guangdong Ocean University (Q15096), and the Department of Education of the Guangdong Province Foundation (2012KJCX0061, 2011-431).

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Authors and Affiliations

  1. Fisheries College, Guangdong Ocean University, Zhanjiang, 524088, China

    Si-Ping Deng, Hua-Pu Chen, Chun-Hua Zhu, Man Ye & Guang-Li Li

  2. Key Laboratory of Marine Ecology and Aquaculture Environment of Zhanjiang, Guangdong Ocean University, Zhanjiang, 524088, China

    Si-Ping Deng, Hua-Pu Chen, Chun-Hua Zhu, Man Ye & Guang-Li Li

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  1. Si-Ping Deng
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Correspondence to Guang-Li Li.

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Deng, SP., Chen, HP., Zhu, CH. et al. Leptin receptor overlapping transcript: characterization, tissue distribution and changes in gene expression in response to eyestalk ablation in the Pacific white shrimp Litopenaeus vannamei . Fish Sci 83, 57–64 (2017). https://doi.org/10.1007/s12562-016-1041-5

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