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Identification of male sex-linked DNA sequence of the cultured Pacific bluefin tuna Thunnus orientalis

  • Original Article
  • Aquaculture
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Abstract

A male-specific marker of a DNA sequence for the Pacific bluefin tuna Thunnus orientalis, Male delta 6 (Md6), was identified by comparative study of the sequences obtained by F3-cultured male and female AFLP-selective DNA amplification products, followed by high-throughput DNA sequencing. Md6 was characteristic in continuous 6-bp nucleotide deletions compared to redundant sequences that could be seen in both sexes. The PCR genotyping primers were developed based on the Md6 sequence, which showed that 29 of 32 F3 males (90.6 %) and 9 of 13 (69.2 %) F2 parental males were genotyping positive, whereas in females, the genotyping test was negative in parental F2 (n = 12), and 1 of 32 (3.1 %) F3 female was Md6-positive. Parentage allocation tests suggested that Md6-positive F2 males were more attendant in spawning than Md6-negative males, suggesting that Md6 was passed from male parents to male progeny under aquaculture conditions.

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Acknowledgments

The authors thank Dr. E. Soeda and Ms. A. Kazama for assistance with AFLP analysis and PBF D-loop primer design. The authors also thank all the staff of the Oshima branch, especially Mr. S. Hayashi, Mr. N. Tsuro, Mr. Y. Hamaguchi, Mr. M. Nakatani, and Mr. T. Okada, for assistance with establishing the PBF blood and DNA specimen bank. This study was supported by a grant from the Japan Science and Technology Agency (JST) SATREPS program and Grant-in-Aid for Young Scientists B #25870937 provided from the Japan Society for the Promotion of Science (JSPS).

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Authors and Affiliations

  1. Oshima Branch, Fisheries Laboratories, Kinki University, 1790-4 Oshima Kushimoto, Wakayama, 649-3633, Japan

    Yasuo Agawa, Mayui Iwaki, Takafumi Komiya, Tomoki Honryo, Kouhei Tamura & Yoshifumi Sawada

  2. Hokkaido System Science Co., Ltd, 1-2-1 Shinkawa-nishi 2 jyo, Kitaku, Sapporo, Hokkaido, 001-0932, Japan

    Tsukasa Okada

  3. Department of Fisheries, Faculty of Agriculture, Kinki University, 3327-204 Nakamachi, Nara, Nara, 631-8505, Japan

    Naoki Yagishita & Toru Kobayashi

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  1. Yasuo Agawa
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  2. Mayui Iwaki
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  3. Takafumi Komiya
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  4. Tomoki Honryo
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  5. Kouhei Tamura
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  7. Naoki Yagishita
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  8. Toru Kobayashi
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  9. Yoshifumi Sawada
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Correspondence to Yasuo Agawa.

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Agawa, Y., Iwaki, M., Komiya, T. et al. Identification of male sex-linked DNA sequence of the cultured Pacific bluefin tuna Thunnus orientalis . Fish Sci 81, 113–121 (2015). https://doi.org/10.1007/s12562-014-0833-8

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  • DOI: https://doi.org/10.1007/s12562-014-0833-8

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