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Genetic characterization of the progeny of a pair of the tetraploid silver crucian carp Carassius auratus langsdorfii

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Abstract

Silver crucian carp Carassius auratus langsdorfii comprises a diploid-polyploid complex in wild Japanese populations. Bisexually reproducing diploids are sympatrically distributed with gynogenetically developing triploids and tetraploids. Triploid and tetraploid males are very rare among Japanese silver crucian carp due to their gynogenetic reproduction. We examined the genetic characteristics of progeny that arose in a tank by natural spawning of a tetraploid silver crucian carp pair. The ploidy status of 120 samples randomly collected from these progeny was determined to be tetraploid by DNA content flow cytometry. DNA fingerprints from a random amplified polymorphic DNA assay indicated that almost all the progeny examined had genotypes identical to the maternal tetraploid female with no paternally derived fragments. Selected specimens’ cytogenetic analyses revealed that the progeny examined had tetraploid chromosome numbers, categorized into 40 metacentric, 80 submetacentric, and 80 subtelocentric or telocentric chromosomes, which were arranged into quartets and six supernumerary microchromosomes. Fluorescence in situ hybridization signals were detected in four homologous chromosomes in all analyzed metaphases prepared from diploid goldfish specimens. Contrary, tetraploid silver crucian carp gave eight rDNA signals. These results suggest that gynogenetic development in eggs spawned by tetraploid females should be triggered by tetraploid males’ homospecific sperm.

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Authors and Affiliations

  1. Faculty and Graduate School of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido, Japan

    Jie Dong, Takafumi Fujimoto & Katsutoshi Arai

  2. School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, Japan

    Masaru Murakami

  3. Nanae Fresh-Water Laboratory, Field Science Center for Northern Biosphere, Hokkaido University, Nanae, Hokkaido, Japan

    Etsuro Yamaha

Authors
  1. Jie Dong
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  2. Masaru Murakami
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  3. Takafumi Fujimoto
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  4. Etsuro Yamaha
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  5. Katsutoshi Arai
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Correspondence to Katsutoshi Arai.

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Dong, J., Murakami, M., Fujimoto, T. et al. Genetic characterization of the progeny of a pair of the tetraploid silver crucian carp Carassius auratus langsdorfii . Fish Sci 79, 935–941 (2013). https://doi.org/10.1007/s12562-013-0674-x

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  • DOI: https://doi.org/10.1007/s12562-013-0674-x

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