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Alterations of pattern in immune response and vitellogenesis during induced ovarian development by unilateral and bilateral ablation in Litopenaeus vannamei

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Abstract

Eyestalk ablation is often used to induce ovarian development in female shrimp, but it possibly alters the animal’s hormonal balance, and affects its physiology and immune system. Therefore, this study was conducted to investigate alterations to the immune response during induced ovarian development by unilateral and bilateral ablation in Litopenaeus vannamei. Sub-adult female shrimp could be induced to undergo ovarian development over a 20-day period by both unilateral and bilateral eyestalk ablation; however, ovaries did not reach the final mature stage in which cortical rods are observed. In terms of immune response, total hemocyte count decreased significantly at 4 h after unilateral ablation, and at 20 days after bilateral ablation. After 20 days, expression in the hemocytes of the immune-related genes prophenoloxidase and peroxinectin in bilaterally ablated shrimp increased significantly, whereas that for penaeidin, for lipopolysaccharide- and β-glucan-binding protein, and for transglutaminase mRNA expression did not change significantly. The results of this study suggest that, while eyestalk ablation induces ovarian development, it may also bring about a hormonal imbalance that leads to immune-related genes being activated.

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Acknowledgments

We are grateful to Ms. Hiroko Endo and Ms. Yoko Furusawa of the Japan International Research Center for Agricultural Sciences (JIRCAS) for their technical assistance.

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Authors and Affiliations

  1. Department of Global Agricultural Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 113-8657, Japan

    Sun-Hye Bae & Marcy N. Wilder

  2. Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, 305-8686, Japan

    Sun-Hye Bae, Tomoyuki Okutsu, Bong Jung Kang & Marcy N. Wilder

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  1. Sun-Hye Bae
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  2. Tomoyuki Okutsu
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  4. Marcy N. Wilder
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Correspondence to Marcy N. Wilder.

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Bae, SH., Okutsu, T., Kang, B.J. et al. Alterations of pattern in immune response and vitellogenesis during induced ovarian development by unilateral and bilateral ablation in Litopenaeus vannamei . Fish Sci 79, 895–903 (2013). https://doi.org/10.1007/s12562-013-0652-3

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  • DOI: https://doi.org/10.1007/s12562-013-0652-3

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