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Identification and expression analyses of MYB and WRKY transcription factor genes in Papaver somniferum L

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Abstract

Papaver somniferum L. is an herbaceous, annual and diploid plant that is important from pharmacological and strategic point of view. The cDNA clones of two putative MYB and WRKY genes were isolated (GeneBank accession numbers KP411870 and KP203854, respectively) from this plant, via the nested-PCR method, and characterized. The MYB transcription factor (TF) comprises 342 amino acids, and exhibits the structural features of the R2R3MYB protein family. The WRKY TF, a 326 amino acid-long polypeptide, falls structurally into the group II of WRKY protein family. Quantitative real-time PCR (qRT-PCR) analyses indicate the presence of these TFs in all organs of P. somniferum L. and Papaver bracteatum L. Highest expression levels of these two TFs were observed in the leaf tissues of P. somniferum L. while in P. bracteatum L. the espression levels were highest in the root tissues. Promoter analysis of the 10 co-expressed gene clustered involved in noscapine biosynthesis pathway in P. somniferum L. suggested that not only these 10 genes are co-expressed, but also share common regulatory motifs and TFs including MYB and WRKY TFs, and that may explain their common regulation.

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Abbreviations

BAC:

Bacterial Artificial Chromosome

E.F.Iα :

Elongation Factor I α

ESTs:

Expressed Sequence Tags

HTH:

Helix-Turn-Helix

ORF:

Open Reading Frame

qRT-PCR:

Quantitative Real-Time PCR

TFBS:

Transcription Factor Binding Site

TSS:

Transcription Start Site

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Acknowledgments

This work was funded by Tarbiat Modares University, Iran. We would like to acknowledge Mahdi Rezaei (University of Tehran, Iran) for providing tissues of P. somniferum and P. bracteatum.

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Authors and Affiliations

  1. Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Tayebeh Kakeshpour, Shadi Nayebi, Sajad Rashidi Monfared, Ahmad Moieni & Ghasem Karimzadeh

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  1. Tayebeh Kakeshpour
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  2. Shadi Nayebi
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  3. Sajad Rashidi Monfared
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  4. Ahmad Moieni
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Correspondence to Sajad Rashidi Monfared.

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Tayebeh Kakeshpour and Shadi Nayebi contributed equally to this work.

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Kakeshpour, T., Nayebi, S., Rashidi Monfared, S. et al. Identification and expression analyses of MYB and WRKY transcription factor genes in Papaver somniferum L. Physiol Mol Biol Plants 21, 465–478 (2015). https://doi.org/10.1007/s12298-015-0325-z

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