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In vivo evaluation of analgesic, anti-inflammatory, and neuropharmacological activities of the chemical constituent from Nepeta clarkei

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Abstract

The plant species of genus Nepeta are used to treat various human diseases and for ornamental purposes as well. Nepethalate B (1) was isolated as a result of phytochemical investigations of Nepeta clarkei and was subjected in the present study for investigation of analgesic, anti-inflammatory, and central nervous system (CNS) depressant activities. The percentage inhibition in phase I of the acetic acid induced writhing test of compound 1 (dose of 0.1, 0.2 and 0.4 mg/kg of body weight) was 53.3, 58.0 and 86.7 % respectively. These results were found significantly higher (P < 0.01) as compared to the negative control. Moreover, the percentage inhibitions of three phases for 0.1, 0.2 and 0.4 mg/kg were higher than the values obtained for Aspirin (positive control). In formalin test, the percentage pain inhibition between 0 and 5 min (early phase) was 68.0, 25.5, and 75.5 % for 0.1, 0.2 and 0.4 mg/kg intra-peritoneal doses of compound 1 respectively. In case of late phase (20–30 min) it was 63.0, 66.7 and 48.1 %, respectively. In comparison to aspirin, overall percentage inhibition of compound 1 was significantly higher in early and late phases. Interestingly, at all doses compound 1 showed more potent anti-inflammatory effects in terms of intensity and duration as compared to aspirin. The gross behavioral study of nepethalate B (1) was also carried out and the results revealed that it exhibited CNS depression in the mice and showed a prominent decrease in locomotor activity.

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Acknowledgments

The authors wish to thank the Higher Education Commission (HEC), Government of Pakistan, for providing financial support for the current study under the National Research Program for Universities (NRPU).

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The authors declare that there is no conflict of interest.

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Authors and Affiliations

  1. Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, Nizwa, 616, Sultanate of Oman

    Javid Hussain, Najeeb Ur Rehman, Ahmed Al-Harrasi, Abdul Latif Khan & Liaqat Ali

  2. National Nematological Research Center, University of Karachi, Karachi, 75270, Pakistan

    Tania Shamim Rizvi

  3. International Center for Chemical and Biological Sciences, H. E. J. Research Institute of Chemistry, University of Karachi, Karachi, 75270, Pakistan

    Faryal Vali Mohammad

  4. Department of Pharmacognosy, Faculty of Pharmacy, Research Institute of Pharmaceutical Sciences, University of Karachi, Karachi, 75270, Pakistan

    Mehjabeen

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  1. Javid Hussain
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  2. Najeeb Ur Rehman
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  3. Ahmed Al-Harrasi
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  4. Abdul Latif Khan
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  7. Mehjabeen
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Correspondence to Javid Hussain or Liaqat Ali.

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Hussain, J., Rehman, N.U., Al-Harrasi, A. et al. In vivo evaluation of analgesic, anti-inflammatory, and neuropharmacological activities of the chemical constituent from Nepeta clarkei . Arch. Pharm. Res. 38, 1188–1194 (2015). https://doi.org/10.1007/s12272-014-0484-4

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  • DOI: https://doi.org/10.1007/s12272-014-0484-4

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