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Effect of Curcumin in Experimental Peritonitis

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Abstract

Despite medical advancements, the inflammatory cascade and oxidative stress worsen the prognosis in most cases of peritonitis. Curcumin has emerged as a potential antioxidant and anti-inflammatory agent in few of the acute inflammatory and infective conditions. We examined the effect of intraperitoneal injection of curcumin in endotoxin-induced peritonitis in rats. The blood and peritoneal fluid samples were collected at 3 and 24 h following the induction of peritonitis. Animals were sacrificed at 24 h and the organs preserved. The histopathological report of lung, liver, and intestines in the curcumin-treated rats showed maintenance of tissue architecture to a large extent compared to the control group which showed massive congestion, hemorrhage, and necrosis. The blood and peritoneal fluid total count and differential neutrophil counts were significantly higher at 24 h of induction of peritonitis. Serum amyloid assay and lipid peroxidation were significantly lower, and myeloperoxidase assay was higher in the curcumin-treated group at the end of 24 h; thus, curcumin probably demonstrated a neutrophil-mediated immunopotentiation and anti-inflammatory action thereby protecting the animal from endotoxemia-induced multi-organ damage.

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Acknowledgments

We thank Dr. J Prakasa Rao for the advice and encouragement. We thank Rajiv Gandhi University of Health Sciences (RGUHS), Karnataka, India for funding the study.

Ethical Approval

All applicable institutional guidelines for the care and use of animals were followed. All procedures performed in studies involving animals were in accordance with the ethical standards of the institution or practice at which the studies were conducted.

Funding

This study was funded by Rajiv Gandhi University of Health Sciences (RGUHS), Karnataka, India (Grant No: RGUHS/R&D/Research Grants/M13/2012-13).

Conflict of Interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Physiology, St. John’s Medical College, John Nagar, Bangalore, 560034, Karnataka, India

    Savitha D

  2. St Johns Research Institute, Karnataka, India

    Indu Mani

  3. Department of Pathology, St. John’s Medical College, Karnataka, India

    Gayatri Ravikumar

  4. Department of Physiology, St. John’s Medical College, Karnataka, India

    Sandhya T Avadhany

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  1. Savitha D
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  2. Indu Mani
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Correspondence to Savitha D.

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D, S., Mani, I., Ravikumar, G. et al. Effect of Curcumin in Experimental Peritonitis. Indian J Surg 77, 502–507 (2015). https://doi.org/10.1007/s12262-015-1303-y

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  • DOI: https://doi.org/10.1007/s12262-015-1303-y

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