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Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation

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Abstract

Objective

This study assessed parameters of free radical damage to biomolecules, mitochondrial superoxide production, superoxide dismutase, and catalase activities and their relationship to sepsis mortality.

Design and setting

Prospective animal study in a university laboratory for experimental.

Subjects

140 male Wistar rats.

Interventions

The animals were randomly divided into three groups: sham-operated (n=20), cecal ligation and perforation resuscitated with normal saline (n=40), and cecal ligation and perforation with normal saline plus antibiotics (n=40).

Measurements and results

Blood samples were collected from all animals 3, 12, and 24 h after CLP through a jugular catheter inserted before CLP. Rats were evaluated during 5 days after the intervention. Nonsurvivor animals were grouped according to the duration between sepsis induction and death, and oxidative parameters were compared to survivors and sham-operated. Lipid peroxidation, protein carbonyls, and superoxide dismutase were significantly increased in nonsurvivor septic rats and were predictive of mortality. We demonstrated that there is a different modulation of superoxide dismutase and catalase in nonsurvivors during the course of septic response. There was a marked increase in superoxide dismutase activity without a proportional increase in catalase activity in nonsurvivors.

Conclusions

This is the first report of plasma superoxide dismutase as an earlier marker of mortality. Ours results might help to clarify an important aspect of oxidative response to sepsis, i.e., an increase in superoxide dismutase activity without a proportional increase in catalase activity

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Acknowledgements

This work was supported by grants from FAPERGS, FIPE-HCPA, and CNPq. The authors thank to Dr. R. Roesler for his critical revision of the manuscript.

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Authors and Affiliations

  1. Departamento de Bioquímica, Centro de Estudos em Estresse Oxidativo, UFRGS, Ramiro Barcelos 2600 anexo, Porto Alegre, 90035-003 Brazil

    Cristiane Ritter, Michael Andrades, Mário Luis C. Frota Jr., Fernanda Bonatto, Ricardo A. Pinho, Manuela Polydoro, Fábio Klamt, José Cláudio F. Moreira & Felipe Dal-Pizzol

  2. Laboratório de Estresse Oxidativo e Doenças, Centro de Pesquisa, HCPA, Porto Alegre, Brazil

    Cristiane Ritter, Michael Andrades, Mário Luis C. Frota Jr., Fernanda Bonatto, Ricardo A. Pinho, Manuela Polydoro, Fábio Klamt, Sérgio S. Menna-Barreto, José Cláudio F. Moreira & Felipe Dal-Pizzol

  3. Serviço de Medicina Intensiva, HCPA, Porto Alegre, Brazil

    Cristiane Ritter & Cleovaldo T. S. Pinheiro

  4. Serviço de Pneumologia, HCPA, Porto Alegre, Brazil

    Sérgio S. Menna-Barreto

  5. Departamento de Medicina, Universidade do Extremo Sul Catarinenese, Porto Alegre, Brazil

    Felipe Dal-Pizzol

Authors
  1. Cristiane Ritter
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  2. Michael Andrades
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  3. Mário Luis C. Frota Jr.
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  4. Fernanda Bonatto
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  5. Ricardo A. Pinho
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  6. Manuela Polydoro
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  7. Fábio Klamt
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  8. Cleovaldo T. S. Pinheiro
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  9. Sérgio S. Menna-Barreto
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  10. José Cláudio F. Moreira
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  11. Felipe Dal-Pizzol
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Corresponding author

Correspondence to Felipe Dal-Pizzol.

Additional information

An editorial regarding this article can be found in the same issue (http://dx.doi.org/10.1007/s00134-003-1861-5)

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Ritter, C., Andrades, M., Frota, M.L.C. et al. Oxidative parameters and mortality in sepsis induced by cecal ligation and perforation. Intensive Care Med 29, 1782–1789 (2003). https://doi.org/10.1007/s00134-003-1789-9

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  • DOI: https://doi.org/10.1007/s00134-003-1789-9

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