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Prolactin and Prolactin Receptor Expression in Cervical Intraepithelial Neoplasia and Cancer

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Pathology & Oncology Research

Abstract

Prolactin receptor (PRLR) overexpression could play a role in tumorigenesis. The aim of this study was to determine prolactin (PRL) and PRLR expression in biopsies from patients with precursor lesions and uterine cervical cancer. PRLR expression was analyzed in 63 paraffin-embedded biopsies of uterine cervical tissue. In total, eleven low-grade squamous intraepithelial lesions (LSIL), 23 high-grade squamous intraepithelial lesions (HSIL), 21 uterine cervical cancers (UCC) and 8 normal epithelium (NE) were examined using immunoperoxidase staining and Western blot analysis. Additionally, PRL expression was identified in human cervical cancer serum and tissues. The PRLR expression was found to be significantly increased in cervical cancer in comparison with normal tissue and precursor lesions (P < 0.0003). The presence of the long isoform of the PRLR was observed only in cervical cancer tissues. Serum PRL levels were normal in all samples and local prolactin expression was similar in precursor lesions and cervical cancer by Western blot analysis. Our data suggest a possible role for PRLR in the progression of cervical cancer.

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Abbreviations

PRL:

prolactin

PRLR:

prolactin receptor

HPV:

human papillomavirus

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Acknowledgments

This work was financed by grants from SEP-CONACYT (79709) and PROMEP UDG-PTC-605.

Authors’ Contributions

RAC and EILP: experimental work; JFMV and CECH: assistance with the writing; ADN, NVS, AGC interpretation of data; STA assistance with ELISAS; RFT, DPM processing samples; ALPS design of the study.

All authors read and approved the final manuscript.

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Authors and Affiliations

  1. Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico

    Rafael Ascencio-Cedillo, Edgar Ivan López-Pulido, Susana Del Toro-Arreola, Adrian Daneri-Navarro & Ana Laura Pereira-Suárez

  2. Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico

    José Francisco Muñoz-Valle

  3. Departamento de Biomedicina Molecular, Centro de Investigación y de Estudios Avanzados-IPN, Unidad Zacatenco, Mexico City, 07360, DF, Mexico

    Nicolás Villegas-Sepúlveda

  4. Centro de Investigación y Asistencia en Tecnología y diseño del Estado de Jalisco A.C, Guadalajara, 44270, Jalisco, Mexico

    Ciro Estrada-Chávez

  5. Departamento de Patología. Nuevo Hospital Civil de Guadalajara, Guadalajara, Jalisco, Mexico

    Ramón Franco-Topete

  6. Departamento de Biología Molecular y Biotecnología, Instituto de Investigaciones Biomédicas UNAM, Mexico City, Mexico

    Delia Pérez-Montiel & Alejandro García-Carrancá

  7. Laboratorio de Inmunología, Departamento de Fisiología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra, Mojada # 950, Colonia Independencia, CP 44340, Guadalajara, Jalisco, Mexico

    Ana Laura Pereira-Suárez

Authors
  1. Rafael Ascencio-Cedillo
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  2. Edgar Ivan López-Pulido
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  3. José Francisco Muñoz-Valle
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  4. Nicolás Villegas-Sepúlveda
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  5. Susana Del Toro-Arreola
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  6. Ciro Estrada-Chávez
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  7. Adrian Daneri-Navarro
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  8. Ramón Franco-Topete
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  11. Ana Laura Pereira-Suárez
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Corresponding author

Correspondence to Ana Laura Pereira-Suárez.

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Ascencio-Cedillo, R., López-Pulido, E.I., Muñoz-Valle, J.F. et al. Prolactin and Prolactin Receptor Expression in Cervical Intraepithelial Neoplasia and Cancer. Pathol. Oncol. Res. 21, 241–246 (2015). https://doi.org/10.1007/s12253-014-9814-6

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  • DOI: https://doi.org/10.1007/s12253-014-9814-6

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