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Drug resistance mutation profiles of the drug-naïve and first-line regimen-treated HIV-1-infected population of Suzhou, China

  • Research Article
  • Published:
Virologica Sinica

Abstract

Little is known about the prevalence of drug-resistant mutations in HIV-1-positive individuals in Suzhou, China. To elucidate the transmitted drug resistance (TDR) and acquired drug resistance mutation (ADR) profiles, we collected blood specimens from 127 drug-naïve and 117 first-line drug-treated HIV-1-infected individuals sampled from 2014 to 2016 in Suzhou. We successfully amplified pol fragments from 100 drug-naïve and 20 drug-treated samples. We then determined the drug-resistant mutations to protease (PR) and reverse-transcriptase (RT) inhibitors according to the Stanford drug resistance database. Overall, 11 and 13 individuals had transmitted (drug-naïve group) and acquired (treated group) resistance mutations, respectively. Six transmitted drug-resistant mutations were found, including two mutations (L33F and L76V) in the protease region and four (K70N/E and V179D/E) in the RT region. Only L76V was a major mutation, and K70N/E and V179D/E are known to cause low-level resistance to RT inhibitors. All 13 treated participants who had major drug resistance mutations demonstrated intermediate to high resistance to efavirenz and nevirapine, and six had a treatment duration of less than three months. No major mutations to RT inhibitors were found, implying that the epidemic of transmitted resistance mutations was not significant in this area. Our results suggest that more frequent virus load and drug resistance mutation tests should be conducted for individuals receiving antiretroviral treatment, especially for newly treated patients. Our research provides insights into the occurrence of HIV-1 drug resistance in Suzhou and will help to optimize the treatment strategy for this population.

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Acknowledgments

This study was supported by grants from the Natural Science Foundation of Jiangsu Province (BL2013017) and the Suzhou Science and Technology Bureau (SYS201156) to Dr. Feng Qian, the Suzhou Health and Family Planning Commission (LCZX201413) to Ming Li, and the Key National Science and Technology Program in the Thirteen Five-Year Plan Period of China (2017ZX10201102-007-002).

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Research Group of HIV-1 Molecular Epidemiology and Virology, The State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Tingting Li, Ting Yuan, Yuying Chen, Rongge Yang & Binlian Sun

  2. University of Chinese Academy of Sciences, Beijing, 100000, China

    Tingting Li & Yuying Chen

  3. Division of HIV-1/AIDS, the Fifth People’s Hospital of Suzhou, Suzhou, 215000, China

    Feng Qian, Weilu Xu, Li Zhu, Jinlong Huang, Haiyan Wang, Yueping Zhu, Yinling Wang, Xiaohong Li, Saihong Gu, Zhuqing Tan, Hui Chen, Xiangrong Luo, Wei Zhu, Wenjuan Lu, Ping Xu, Ming Li & Chuanwu Zhu

  4. Department of Microbiology and Immunology, Schulich School of Medicine & Dentistry, University of Western Ontario, London, N6A 5C1, Canada

    Yong Gao

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  1. Tingting Li
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  2. Feng Qian
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Contributions

TL, FQ, TY, RY, YG, CZ and BS conceived the experiments; WX, LZ, JH, HW, YZ, YW, XL, SG, ZT, XL, WZ, PX, HC, WL and ML collected the samples; TL and YC performed the experiments; TL analyzed the data; TL, FQ and BS wrote and finalized the manuscript. All authors have read and approved the final manuscript.

Corresponding authors

Correspondence to Chuanwu Zhu or Binlian Sun.

Ethics declarations

The authors declare that there is no conflict of interest. The study was approved by the Ethics Committees of the Fifth People’s Hospital of Suzhou and Wuhan Institute of Virology. Informed consent was obtained from all participants in this study.

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12250_2017_4002_MOESM1_ESM.pdf

Drug resistance mutation profiles of the drug-naïve and first-line regimen-treated HIV-1-infected population of Suzhou, China

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Li, T., Qian, F., Yuan, T. et al. Drug resistance mutation profiles of the drug-naïve and first-line regimen-treated HIV-1-infected population of Suzhou, China. Virol. Sin. 32, 271–279 (2017). https://doi.org/10.1007/s12250-017-4002-y

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