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Preparation and characterization of monoclonal antibodies against VP1 protein of foot-and-mouth disease virus O/China99

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Virologica Sinica

Abstract

Monoclonal antibodies (McAbs) 1A9 and 9F12 against Foot-and-mouth disease virus (FMDV) serotype O were produced by fusing SP2/0 myeloma cells with splenocyte from the mouse immunized with O/China99. Both McAbs reacted with O/China99 but not with Asia 1, as determined by immunohistochemistry assay. The microneutralization titer of the McAbs 1A9 and 9F12 were 640 and 1 280, respectively. Both McAbs contain kappa light chains, but the McAbs 1A9 and 9F12 were IgG1 and IgM, respectively. In order to define the McAbs binding epitopes, the reactivity of these McAbs against VP1, P20 and P14 were examined using indirect ELISA, the result showed that both McAbs reacted with VP1 and P20. McAbs may be used for further studies of vaccine, diagnostic methods, prophylaxis, etiological and immunological researches on FMDV.

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Authors and Affiliations

  1. National FMD Reference Laboratory, Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricutral Science, Lanzhou, 730046, China

    Shuai Song, Tong Lin, Jun-jun Shao, Shan-dian Gao, Guo-zheng Cong, Jun-zheng Du & Hui-yun Chang

  2. Guangdong Open Laboratory of Veterinary Public Healty, Institute of Veterinary Medicine, Guangdong Academy of Agricultural Science, Guangzhou, 510640, China

    Shuai Song

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  1. Shuai Song
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  2. Tong Lin
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  4. Shan-dian Gao
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Correspondence to Hui-yun Chang.

Additional information

Foundation items: The national high technology research and development program of China 863 (2006AA10A 204); The national science and technology pillar program (2006BAD06A17).

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Song, S., Lin, T., Shao, Jj. et al. Preparation and characterization of monoclonal antibodies against VP1 protein of foot-and-mouth disease virus O/China99. Virol. Sin. 24, 566–572 (2009). https://doi.org/10.1007/s12250-009-3061-0

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  • DOI: https://doi.org/10.1007/s12250-009-3061-0

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