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Fed-Batch Fermentation for Spinosad Production in an Improved Reactor

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Abstract

As a kind of aerobic bacteria, Saccharopolyspora spinosa exhibits a high demand for oxygen. In the fermentation process, the methods of increasing ventilation and improving agitation speed are usually adopted to achieve higher values of dissolved oxygen. These methods decrease the efficiency of spinosad biosynthesis. In this study, an improved reactor was designed to solve these problems. The exhaust gas reflux device, impellers, and baffles were improved. Furthermore, we established the kinetic models for the cell growth, substrate consumption and spinosad generation in batch fermentation process. The simulation results were in good agreement with the experimental data. Spinosad production reached 583.86 mg/L after employing the suitable feeding strategy by fed-batch fermentation in the improved reactor, whereas it was only 157.01 mg/L before optimization. The method described can provide insight to strengthen spinosad production and can be extended to the culturing process of filamentous aerobic bacteria.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 21076148 and No. 31270087); and Plan of Tianjin Science and Technology Support (11ZCKFSY0100).

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Authors and Affiliations

  1. School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, China

    Chunzhe Lu, Jing Yin, Chuanbo Zhang & Wenyu Lu

  2. Key Laboratory of System Bioengineering, Ministry of Education, Tianjin, 300072, China

    Chunzhe Lu, Jing Yin, Chuanbo Zhang & Wenyu Lu

  3. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Tianjin, 300072, China

    Chunzhe Lu, Jing Yin, Chuanbo Zhang & Wenyu Lu

Authors
  1. Chunzhe Lu
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  2. Jing Yin
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  3. Chuanbo Zhang
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  4. Wenyu Lu
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Correspondence to Chunzhe Lu.

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Lu, C., Yin, J., Zhang, C. et al. Fed-Batch Fermentation for Spinosad Production in an Improved Reactor. Trans. Tianjin Univ. 23, 530–537 (2017). https://doi.org/10.1007/s12209-017-0062-1

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  • DOI: https://doi.org/10.1007/s12209-017-0062-1

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