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Electrochemical behavior of isometamidium and its determination in milk at a SWCNT/AuNP-modified electrode

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Abstract

Isometamidium (ISM) residues in milk can cause health problems to human beings. This manuscript described for the first time an electrochemical method for ISM analysis in milk samples. The voltammetric behavior of ISM over an extended pH range using both cyclic voltammetry and differential pulse voltammetry at a glassy carbon electrode (GCE) was also studied. In order to improve the analytical performance, GC electrodes were modified with aniline and 1,4-phenylenediamine to form a stable layer, and then single-walled carbon nanotubes (SWCNT) were covalently bound to the GC electrodes. Gold nanoparticles (AuNP) were electrodeposited to the GC electrodes through the C–Au bonds to further promote the electrode conductivity. The modified SWCNT/AuNP-GCE electrochemical sensor (EC) was successfully applied to analyze isometamidium residues in milk. The linear range was from 10 μg to 400 μg/L with limit of detection at 3 μg/L (S/N = 3). The recovery was between 79.4–94.7 % in spiked samples. The EC sensor method was also compared with a LC-MS/MS method, which proves its capability in commercial market surveillance.

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Acknowledgments

Funding

This work was supported by the Natural Science Foundation of China (31371779) and the International Science & Technology Cooperation Program of China (2015DFG31890).

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Authors and Affiliations

  1. Key Laboratory of Agro-Product Quality and Safety, Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences (CAAS), 100081, Beijing, China

    Dan Zhu, Qiangqiang Li, Xiumei Pang & Gang Chen

  2. Institute of Quality and Standards for Agricultural Products, Zhejiang Academy of Agricultural Sciences, 310014, Hangzhou, China

    Yuwei Yuan

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  1. Dan Zhu
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  2. Qiangqiang Li
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  3. Xiumei Pang
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  4. Yuwei Yuan
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Correspondence to Yuwei Yuan or Gang Chen.

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Dan Zhu declares that she has no conflict of interest. Qiangqiang Li declares that she has no conflict of interest. Xiumei Pang declares that she has no conflict of interest. Yuwei Yuan declares that he has no conflict of interest. Gang Chen declares that he has no conflict of interest.

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Zhu, D., Li, Q., Pang, X. et al. Electrochemical behavior of isometamidium and its determination in milk at a SWCNT/AuNP-modified electrode. Food Anal. Methods 9, 1963–1969 (2016). https://doi.org/10.1007/s12161-015-0376-3

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  • DOI: https://doi.org/10.1007/s12161-015-0376-3

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