Article

Biomolecular NMR Assignments

, Volume 7, Issue 2, pp 315-319

Open Access This content is freely available online to anyone, anywhere at any time.

1H, 13C and 15N resonance assignments of human BASP1

  • Leonhard GeistAffiliated withDepartment of Computational and Structural Biology, Max F. Perutz Laboratories, University of Vienna
  • , Anna Zawadzka-KazimierczukAffiliated withFaculty of Chemistry, University of Warsaw
  • , Saurabh SaxenaAffiliated withFaculty of Chemistry, University of Warsaw
  • , Szymon ŻerkoAffiliated withFaculty of Chemistry, University of Warsaw
  • , Wiktor KoźmińskiAffiliated withFaculty of Chemistry, University of Warsaw
  • , Robert KonratAffiliated withDepartment of Computational and Structural Biology, Max F. Perutz Laboratories, University of Vienna Email author 

Abstract

Brain acid-soluble protein 1 (BASP1, CAP-23, NAP-22) appears to be implicated in diverse cellular processes. An N-terminally myristoylated form of BASP1 has been discovered to participate in the regulation of actin cytoskeleton dynamics in neurons, whereas non-myristoylated nuclear BASP1 acts as co-suppressor of the potent transcription regulator WT1 (Wilms’ Tumor suppressor protein 1). Here we report NMR chemical shift assignment of recombinant human BASP1 fused to an N-terminal cleavable His6-tag.

Keywords

BASP1 NMR signal assignment Intrinsically disordered protein Myc oncogene WT1