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Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease

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Abstract

An impedimetric mga gene specific DNA sensor was developed by immobilization of single stranded DNA probe onto the screen printed modified gold-dendrimer nanohybrid composite electrode for early and rapid detection of S. pyogenes in human throat swab samples causing rheumatic heart disease. Electrochemical impedance response was measured after hybridization with bacterial single stranded genomic DNA (ssG-DNA) with probe. The sensor was found highly specific to S. pyogenes and can detect as low as 0.01 ng ssDNA in 6 µL sample only in 30 min. The nanohybrid sensor was also tested with non-specific pathogens and characterized by FTIR. An early detection of the pathogen S. pyogenes in human can save damage of mitral and aortic heart valves (rheumatic heart disease) by proper medical care.

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References

  1. Guilherme L, Köhler KF, Postol E, Kalil J (2011) Genes, autoimmunity and pathogenesis of rheumatic heart disease. Ann Pediatr Cardiol 4:13–21. doi:10.4103/0974-2069.79617

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Dajani AS, Ayoub EM, Bierman FZ, Bisno AL, Deny FW et al (1993) Guidelines for the diagnosis of rheumatic fever: Jones criteria. Circulation 87:302–307. doi:10.1002/14651858.CD002227

    Article  Google Scholar 

  3. Carapetis JR, McDonald M, Wilson NJ (2005) Acute rheumatic fever. Lancet 366:155–168. doi:10.1016/S0140-6736(05)66874-2

    Article  PubMed  Google Scholar 

  4. Guilherme L, Faé K, Oshiro SE, Kalil J (2005) Molecular pathogenesis of rheumatic fever and rheumatic heart disease. Expert Rev Mol Med 7:1–15. doi:10.1017/S146239940501015X

    Article  PubMed  Google Scholar 

  5. Pedro MA, Rosa RP, Luiza G (2012) Understanding rheumatic fever. Rheumatol Int 32:1113–1120. doi:10.1007/s00296-011-2152-z

    Article  Google Scholar 

  6. Kalia VC, Kumar R, Kumar P, Koul S (2016) A genome-wide profiling strategy as an aid for searching unique identification biomarkers for Streptococcus. Indian J Microbiol 56:46–58. doi:10.1007/s12088-015-0561-5

    Article  CAS  PubMed  Google Scholar 

  7. Kumar R, Koul S, Kumar P, Kalia VC (2016) Searching biomarkers in the sequenced genomes of Staphylococcus for their rapid identification. Indian J Microbiol 56:64–71. doi:10.1007/s12088-016-0565-9

    Article  CAS  PubMed  Google Scholar 

  8. Kalia VC, Kumar P (2015) Genome wide search for biomarkers to diagnose Yersinia infections. Indian J Microbiol 55:366–374. doi:10.1007/s12088-015-0552-6

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Kalia VC, Kumar P, Kumar R, Mishra A, Koul S (2015) Genome wide analysis for rapid identification of Vibrio species. Indian J Microbiol 55:375–383. doi:10.1007/s12088-015-0553-5

    Article  PubMed  PubMed Central  Google Scholar 

  10. Kekre A, Bhushan A, Kumar P, Kalia VC (2015) Genome wide analysis for searching novel markers to rapidly identify Clostridium strains. Indian J Microbiol 55:250–257. doi:10.1007/s12088-015-0535-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Koul S, Kalia VC (2016) Comparative genomics reveals biomarkers to identify Lactobacillus species. Indian J Microbiol 56:253–263. doi:10.1007/s12088-016-0605-5

    Google Scholar 

  12. Koul S, Kumar P, Kalia VC (2015) A unique genome wide approach to search novel markers for rapid identification of bacterial pathogens. J Mol Genet Med 9:194. doi:10.4172/1747-0862.1000194

    Article  Google Scholar 

  13. Uhl JR, Patel RB (2016) Fifteen-minute detection of Streptococcus pyogenes in throat swabs by use of a commercially available point-of-care PCR assay. J Clin Microbiol 54(3):815–817. doi:10.1128/JCM.03387-15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Singh S, Kaushal A, Khare S, Kumar A (2014) Gold–mercaptopropionic acid–polyethylenimine composite based DNA sensor for early detection of rheumatic heart disease. Analyst 139:3600–3606. doi:10.1039/C4AN00324A

    Article  CAS  PubMed  Google Scholar 

  15. Singh S, Kaushal A, Khare S, Kumar A (2014) mga genosensor for early detection of human rheumatic heart disease. Appl Biochem Biotechnol 173:228–238. doi:10.1007/s12010-014-0836-z

    Article  CAS  PubMed  Google Scholar 

  16. Dash SK, Sharma M, Khare S, Kumar A (2012) Quick diagnosis of human brain meningitis using Omp85 gene amplicon as a genetic marker. Indian J Microbiol 53(2):238–240. doi:10.1007/s12088-013-0371-6

    Article  Google Scholar 

  17. Hui P, Lijuan Z, Christian S, Jadranka TS (2009) Conducting polymers for electrochemical DNA sensing. Biomaterials 30:2132–2148. doi:10.1016/j.biomaterials.2008.12.065

    Article  Google Scholar 

  18. Kumar A, Dash SK, Sharma DP, Suman (2012) DNA based biosensors for detection of pathogens. In: Singh HP, Chowdappa P, Chakroborty BN, Podie AR (eds) Molecular approaches for plant fungal disease management. Westville Publishers, Delhi, pp 31–35

  19. Sheehan J, Cruickshank P, Boshart G (1961) A convenient synthesis of water-soluble carbodiimide. J Org Chem 26:2525–2528. doi:10.1021/jo01351a600

    Article  CAS  Google Scholar 

  20. Yang T, Guo X, Ma Y, Li Q, Zhong L, Jiao K (2013) Electrochemical impedimetric DNA sensing based on multi-walled carbon nanotubes-SnO2-chitosan nanocomposite. Colloids Surf B Biointerfaces 107:257–261. doi:10.1016/j.colsurfb.2013.01.046

    Article  CAS  PubMed  Google Scholar 

  21. Park JY, Park SM (2009) DNA hybridization sensors based on electrochemical impedance spectroscopy as a detection tool. Sensors 9:9513–9532. doi:10.3390/s91209513

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

Ms. Swati Singh thanks Council of Scientific and Industrial Research, New Delhi for providing financial assistance (CSIR-SRF) to carry out the research work. Authors also thank to Prof. N. N. Mathur (ENT), Safdarjung Hospital for providing patient samples.

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Authors and Affiliations

  1. CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India

    Swati Singh, Ankur Kaushal & Ashok Kumar

  2. Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110025, India

    Swati Singh & Ashok Kumar

  3. National Centre for Disease Control, Sham Nath Marg, Delhi, 110054, India

    Sunil Gupta

Authors
  1. Swati Singh
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  2. Ankur Kaushal
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  3. Sunil Gupta
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  4. Ashok Kumar
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Corresponding author

Correspondence to Ashok Kumar.

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The authors declare that they have no conflict of interest.

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Singh, S., Kaushal, A., Gupta, S. et al. Gene Specific Impedimetric Bacterial DNA Sensor for Rheumatic Heart Disease. Indian J Microbiol 57, 112–115 (2017). https://doi.org/10.1007/s12088-016-0620-6

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  • DOI: https://doi.org/10.1007/s12088-016-0620-6

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