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mga Genosensor for Early Detection of Human Rheumatic Heart Disease

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Abstract

The 5′ amino-labeled DNA probe complementary to mga gene of Streptococcus pyogenes was immobilized on carboxylated multiwall carbon nanotubes electrode and hybridized with 0.1–100 ng/6 μl single-stranded genomic DNA (ssG-DNA) of S. pyogenes from throat swab of suspected rheumatic heart disease (RHD) patients. Electrochemical response was measured by cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance (EI). The sensitivity of the sensor was 106.03 (μA/cm2)/ng and limit of detection (LOD) was found 0.014 ng/6 μl with regression coefficient (R 2) of 0.921 using DPV. The genosensor was characterized by FTIR and SEM, and electrode was found stable for 6 months on storage at 4 °C with 5–6 % loss in initial DPV current. mga genosensor is the first report on RHD sensor which can save life of several suspected patients by early diagnosis in 30 min.

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References

  1. Fox, J. W., Marcon, M. J., & Bonsu, B. K. (2006). Journal of Clinical Microbiology, 44(7), 2593–2594.

    Article  Google Scholar 

  2. Kaushal, A., Goyal, L., & Kumar, A. (2011). Bioscience Biotechnology Research Communications, 4(1), 1–6.

    CAS  Google Scholar 

  3. Stevens, D. L., & Kaplan, E. L. (2000). Streptococcal infections: Clinical aspects, microbiology and molecular pathogenesis (1st ed.). USA: Oxford University Press.

    Google Scholar 

  4. Ogay, K., & Bille, J. (1986). European Journal of Clinical Microbiology, 5(3), 317–319.

    Article  CAS  Google Scholar 

  5. Dunne, E. M., Marshall, J. L., Baker, C. A., Manning, J., Gonis, G., Danchin, M. H., et al. (2013). BMC Infectious Diseases, 13, 312–317.

    Article  CAS  Google Scholar 

  6. Borek, A. L., Obszańska, K., Hryniewicz, W., & Sitkiewicz, I. (2012). Virulence, 3(6), 529–533.

    Article  Google Scholar 

  7. Anderson, N. W., Buchan, B. W., Mayne, D., Mortensen, J. E., Mackey, T. L., & Ledeboer, N. A. (2013). Journal of Clinical Microbiology, 51(5), 1474–1477.

    Article  Google Scholar 

  8. Tajbakhsh, S., Gharibi, S., Zandi, K., Yaghobi, R., & Asayesh, G. (2011). European Review for Medical and Pharmacological Sciences, 15(3), 313–317.

    CAS  Google Scholar 

  9. Ahmed, A., Rushworth, J. V., Wright, J. D., & Millner, P. A. (2013). Analytical Chemistry, 85(24), 12118–12125.

    Article  CAS  Google Scholar 

  10. Kaushal, A., Kumar, D., Khare, S., & Kumar, A. (2012). Cell and Molecular Biology, 58, 50–54.

    CAS  Google Scholar 

  11. Goyal, K., Chitransh, R., Khare, S., Chaudhary, R., & Kumar, A. (2012). Journal of Bioscience and Medicine, 2, 1–5.

    Google Scholar 

  12. Kumar, A., Bhatnagar, A., Gupta, S., Khare, S., & Suman. (2012). Cell and Molecular Biology, 57, 26–30.

    Google Scholar 

  13. Yang, S., & Rothman, R. E. (2004). The Lancet, 4(6), 337–348.

    Article  CAS  Google Scholar 

  14. Gerber, M. A., Baltimore, R. S., Eaton, C. B., Gewitz, M., Rowley, A. H., Shulman, S. T., et al. (2009). Circulation, 119, 1541–1551.

    Article  CAS  Google Scholar 

  15. Kachoosangi, R. T., Wildgoose, G. G., & Compton, R. G. (2008). Electroanalysis, 15, 1714–1718.

    Article  Google Scholar 

  16. Mciver, K. S., Thurman, A. S., & Scott, J. R. (1999). Journal of Bacteriology, 181(17), 5373–5383.

    CAS  Google Scholar 

  17. Hondorp, E. R., & McIver, K. S. (2007). Molecular Microbiology, 66(5), 1056–1065.

    Article  CAS  Google Scholar 

  18. Louie, M., Louie, L., & Simor, A. E. (2000). Canadian Medical Association Journal, 63(3), 301–309.

    Google Scholar 

  19. Karadeniz, H., Erdem, A., & Caliskan, A. (2008). Electroanalysis, 20(17), 1932–1938.

    Article  CAS  Google Scholar 

  20. Mulchandani, A., & Rajesh, R. (2011). Applied Biochemistry and Biotechnology, 165, 687–699.

    Article  CAS  Google Scholar 

  21. Garcia-Aljaro, C., Cella, L. N., Shirale, D. J., Park, M., Munoj, F. J., Yates, M. V., et al. (2010). Biosensors and Bioelectronics, 26, 1437–1441.

    Article  CAS  Google Scholar 

  22. Sarkar, T., Gao, Y. N., & Mulchandani, A. (2013). Applied Biochemistry and Biotechnology, 170, 1011–1025.

    Article  CAS  Google Scholar 

  23. Gong, J. L., Sarkar, T., Badhulika, S., & Mulchandani, A. (2013). Applied Physics Letters, 102, 13701–13703.

    Article  Google Scholar 

  24. Mulchandani, A. & Rogers, K. (2012). Enzyme and microbial biosensors: Technique and protocols, Vol. 6: Methods in Biotechnology (Walker, J. M., ed.), Humana, Totowa, NJ, pp. 284

  25. Sims, M. J., Li, Q., Kachoosangi, R. T., Wildgoose, G. G., & Compton, R. G. (2009). Electrochimica Acta, 54, 5030–5034.

    Article  CAS  Google Scholar 

  26. Liu, L., Li, S., Liu, L., Deng, D., & Xia, N. (2012). Analyst, 137, 3794–3799.

    Article  CAS  Google Scholar 

  27. Rahman, M. M., Shiddiky, M. J. A., Rahman, M. A., & Shim, Y. B. (2009). Analytical Biochemistry, 384, 159–165.

    Article  CAS  Google Scholar 

  28. Zhu, L., Zhao, R., Wang, K., Xiang, H., Shang, Z., & Sun, W. (2008). Sensors, 8, 5649–5660.

    Article  CAS  Google Scholar 

  29. Nasef, H., Beni, V., & O’Sullivan, C. K. (2010). Analytical and Bioanalytical Chemistry, 396(4), 1423–1432.

    Article  CAS  Google Scholar 

  30. Yang, T., Guo, X., Ma, Y., Li, Q., Zhong, L., & Jiao, K. (2013). Colloids and Surfaces B, 107(1), 257–261.

    Article  CAS  Google Scholar 

  31. Park, J., & Park, S. (2009). Sensors, 12, 9513–9532.

    Article  Google Scholar 

  32. Marty, R., Kossi, C. N. N., Charbonneau, D., Wienert, C. M., Karepalk, L., & Riahi, H. A. T. (2009). Nucleic Acids Research, 37, 849–857.

    Article  CAS  Google Scholar 

  33. Malins, D. C., Gilman, N. K., Green, V. M., Wheeler, T. M., Barker, E. A., & Anderson, K. M. (2005). Proceedings of National Academy of Sciences, 102, 19093–19096.

    Article  CAS  Google Scholar 

  34. Dash, S. K., Sharma, M., Khare, S., & Kumar, A. (2013). Indian Journal of Microbiology. doi:10.1007/s 12088-013-0435-7.

    Google Scholar 

  35. Balaji, K., Thenmozhi, R., Sundaravadivel, M., & Pandian, S. K. (2012). Applied Biochemistry and Biotechnology, 167, 1459–1473.

    Article  CAS  Google Scholar 

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Acknowledgments

Swati Singh thanks CSIR, New Delhi for providing fellowship to carry out the research work.

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Authors and Affiliations

  1. CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India

    Swati Singh, Ankur Kaushal & Ashok Kumar

  2. National Centre for Disease Control, Sham Nath Marg, Delhi, 110054, India

    Shashi Khare

  3. Academy of Scientific and Innovative Research (AcSIR), New Delhi, India

    Swati Singh & Ashok Kumar

Authors
  1. Swati Singh
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  2. Ankur Kaushal
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  3. Shashi Khare
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  4. Ashok Kumar
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Correspondence to Ashok Kumar.

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Singh, S., Kaushal, A., Khare, S. et al. mga Genosensor for Early Detection of Human Rheumatic Heart Disease. Appl Biochem Biotechnol 173, 228–238 (2014). https://doi.org/10.1007/s12010-014-0836-z

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  • DOI: https://doi.org/10.1007/s12010-014-0836-z

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