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Different expression and subcellular localization of Phosphoinositide-specific Phospholipase C enzymes in differently polarized macrophages

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Journal of Cell Communication and Signaling Aims and scope

Abstract

Macrophages’ phenotypic and functional diversity depends on differentiating programs related to local environmental factors. Recent interest was deserved to the signal transduction pathways acting in macrophage polarization, including the phosphoinositide (PI) system and related phospholipase C (PLC) family of enzymes. The expression panel of PLCs and the subcellular localization differs in quiescent cells compared to the pathological counterpart. We analyzed the expression of PLC enzymes in unpolarized (M0), as well as in M1 and M2 macrophages to list the expressed isoforms and their subcellular localization. Furthermore, we investigated whether inflammatory stimulation modified the basal panel of PLCs’ expression and subcellular localization. All PLC enzymes were detected within both M1 and M2 cells, but not in M0 cells. M0, as well as M1 and M2 cells own a specific panel of expression, different for both genes’ mRNA expression and intracellular localization of PLC enzymes. The panel of PLC genes’ expression and PLC proteins’ presence slightly changes after inflammatory stimulation. PLC enzymes might play a complex role in macrophages during inflammation and probably also during polarization.

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    Authors and Affiliations

    1. Department of Medico-Surgical Sciences and Biotechnology, Polo Pontino – Sapienza University, Latina, Italy

      Tania Di Raimo, Martina Leopizzi, Giorgio Mangino, Carlo Della Rocca & Rita Businaro

    2. Department Sensory Organs, Faculty of Medicine and Dentistry, Policlinico Umberto I, Sapienza University of Rome, Viale dell’Università 33, 00100, Rome, Italy

      Lucia Longo & Vincenza Rita Lo Vasco

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    1. Tania Di Raimo
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    2. Martina Leopizzi
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    3. Giorgio Mangino
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    4. Carlo Della Rocca
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    5. Rita Businaro
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    6. Lucia Longo
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    7. Vincenza Rita Lo Vasco
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    Correspondence to Vincenza Rita Lo Vasco.

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    Tania Di Raimo and Martina Leopizzi contributed equally to this work.

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    Di Raimo, T., Leopizzi, M., Mangino, G. et al. Different expression and subcellular localization of Phosphoinositide-specific Phospholipase C enzymes in differently polarized macrophages. J. Cell Commun. Signal. 10, 283–293 (2016). https://doi.org/10.1007/s12079-016-0335-9

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