ABSTRACT
Type VII Secretion System (T7SS) is one of the factors involved in virulence of Mycobacteriun tuberculosis H37Rv. Numerous research efforts have been made in the last decade towards characterizing the components of this secretion system. An extensive genome-wide analysis through compilation of isolated information is required to obtain a global view of diverse characteristics and pathogenicity-related aspects of this machinery. The present study suggests that differences in structural components (of T7SS) between Actinobacteria and Firmicutes, observed earlier in a few organisms, is indeed a global trend. A few hitherto uncharacterized T7SS-like clusters have been identified in the pathogenic bacteria Enterococcus faecalis, Saccharomonospora viridis, Streptococcus equi, Streptococcuss gordonii and Streptococcus sanguinis. Experimental verification of these clusters can shed lights on their role in bacterial pathogenesis. Similarly, verification of the identified variants of T7SS clusters consisting additional membrane components may help in unraveling new mechanism of protein translocation through T7SS. A database of various components of T7SS has been developed to facilitate easy access and interpretation of T7SS related data.
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Abbreviations
- HMM:
-
Hidden Marcov Model
- T7SS:
-
Type VII Secretion System
- T7SSC:
-
Type VII Secretion System Component
- T7CD:
-
Type VII Secretion System Component Database
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Acknowledgments
We thank Hemang Gandhi for development of the web interface. CD is also a PhD student at the Department of Chemical Engineering, Indian Institute of Technology (IIT), Bombay, and would like to acknowledge the department for its support.
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[Das C, Ghosh TS and Mande SS 2016 In silico dissection of Type VII Secretion System components across bacteria: New directions towards functional characterization. J. Biosci.] DOI 10.1007/s12038-016-9599-8
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Das, C., Ghosh, T.S. & Mande, S.S. In silico dissection of Type VII Secretion System components across bacteria: New directions towards functional characterization. J Biosci 41, 133–143 (2016). https://doi.org/10.1007/s12038-016-9599-8
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DOI: https://doi.org/10.1007/s12038-016-9599-8