Abstract
Trans-acting small interfering RNAs (ta-siRNAs) are a class of endogenous small RNA, associated with post-transcriptional gene silencing. Their biogenesis requires an initial microRNA (miRNA)-mediated cleavage of precursor RNA. Around 20 different ta-siRNA-producing loci (TASs), whose sequences are conserved, are reported in plants. In tomato, two TAS gene families have been identified, which are found to target auxin response factor gene and bacterial spot disease resistance protein Bs4 gene. Using high-throughput computational and experimental approach, we identified a new locus-producing ta-siRNA in tomato. We have also identified the putative miRNA regulating the production of ta-siRNA from this locus. The ta-siRNAs generated from TAS4 were up-regulated upon infection with a DNA virus. The potential targets of ta-siRNAs were predicted to be variety of proteins including MYB transcription factors and cell cycle regulators for some of the ta-siRNAs produced.
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AS and SS are thankful to University Grants Commission, New Delhi, and Department of Biotechnology, India, respectively, for their research fellowships.
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[Singh A, Saraf S, Dasgupta I and Mukherjee SK 2016 Identification and validation of a virus-inducible ta-siRNA-generating TAS4 locus in tomato. J. Biosci.] DOI 10.1007/s12038-016-9590-4
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Singh, A., Saraf, S., Dasgupta, I. et al. Identification and validation of a virus-inducible ta-siRNA-generating TAS4 locus in tomato. J Biosci 41, 109–118 (2016). https://doi.org/10.1007/s12038-016-9590-4
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DOI: https://doi.org/10.1007/s12038-016-9590-4