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Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay

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Abstract

Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay. Our results indicated that hMSCA-CM improved cell viability, reduced nuclear fragmentation, attenuated the production of reactive oxygen species, and preserved mitochondrial membrane potential and ultrastructural parameters. In addition, hMSCA-CM upregulated neuroglobin in T98G cells and the genetic silencing of this protein prevented the protective action of hMSCA-CM on damaged cells, suggesting that neuroglobin is mediating, at least in part, the protective effect of hMSCA-CM. Overall, this evidence suggests that the use of hMSCA-CM is a promising therapeutic strategy for the protection of astrocytic cells in central nervous system (CNS) pathologies.

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Acknowledgments

The authors thank Dr. Jorge Andres Afanador and the staff of the cosmetic surgery Clinic DHARA in Bogotá - Colombia, for the adipose tissue samples. This work was supported by PUJ grant #6260 to GEB and scholarship for doctoral studies awarded by the Vicerrectoria Académica of PUJ to Baez-Jurado E.

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Authors and Affiliations

  1. Departamento de Nutrición y Bioquímica, Facultad de Ciencias, Pontificia Universidad Javeriana, Bogotá, DC, Colombia

    Eliana Baez-Jurado, Gina Guio Vega & George E. Barreto

  2. Institute of Physiologically Active Compounds Russian Academy of Sciences, Chernogolovka, 142432, Russia

    Gjumrakch Aliev

  3. GALLY International Biomedical Research Consulting LLC, San Antonio, TX, 78229, USA

    Gjumrakch Aliev

  4. School of Health Science and Healthcare Administration, University of Atlanta, Johns Creek, GA, 30097, USA

    Gjumrakch Aliev

  5. Institute of Pharmacy and Translational Medicine, Sechenov First Moscow State Medical University, 2-4 Bolshaya Pirogovskaya st., 119991, Moscow, Russia

    Vadim V. Tarasov

  6. Facultad Medicina Veterinaria y Zootecnia, Universidad Nacional de Colombia, Bogotá, Colombia

    Paula Esquinas

  7. Facultad Ciencias de la Salud, Universidad San Sebastián, Lientur 1457, 4030000, Concepción, Chile

    Valentina Echeverria

  8. Instituto de Ciencias Biomédicas, Universidad Autónoma de Chile, Santiago, Chile

    George E. Barreto

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  1. Eliana Baez-Jurado
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Correspondence to George E. Barreto.

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Baez-Jurado, E., Vega, G.G., Aliev, G. et al. Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay. Mol Neurobiol 55, 2285–2300 (2018). https://doi.org/10.1007/s12035-017-0481-y

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