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Effect of 6′-acetylpaeoniflorin on dinitrochlorobenzene-induced allergic contact dermatitis in BALB/c mice

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Abstract

Allergic contact dermatitis (ACD) is a classical experimental model of allergic inflammatory skin disease, which is a delayed-type hypersensitivity reaction that is mediated by hapten-specific T lymphocytes. The goal of this study was to investigate the pharmacokinetic profiles of 6′-acetylpaeoniflorin (6-AP) and the effect of 6-AP on the ACD model. 6-AP was synthesized from paeoniflorin (Pae) via acetylation, and the structure was confirmed. There were statistically significant differences in the pharmacokinetic parameters including t 1/2α , t 1/2β , AUC, MRT and C max among the animals that were orally administered Pae and 6-AP. An ACD model was induced using immunization with dinitrochlorobenzene (DNCB) in BALB/c mice. The mice were orally administered 6-AP (35, 70 and 140 mg/kg/d), Pae (70 mg/kg/d) and prednisone (Pre, 5 mg/kg/d) from day 1 to day 7 after immunization. The results indicated that the topical application of DNCB to the skin provoked obvious inflammatory responses. 6-AP significantly inhibited ear swelling and decreased inflammatory cell infiltration and epidermal keratinization. Additionally, 6-AP observably alleviated the hyperplasia of red pulp and germinal center appearance, decreased the spleen index and inhibited splenocyte proliferation in the ACD model compared to that of Pae. Furthermore, the study indicated that 6-AP could increase the IL-10 level, while simultaneously reducing the IL-17 level in splenocytes. In summary, these results suggest that 6-AP has a significantly higher anti-inflammatory effect than Pae and that 6-AP might be a candidate for the treatment of allergic skin diseases.

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Acknowledgments

This work was financially supported by the National Natural Science Foundation of China (Nos. 81330081, 81302845, 81473223), the Training programme of Academic and technical leaders in universities of Anhui province (No. 34), the Anhui Province Nature Science Foundation in University (No. KJ2013A158) and the Grants for Scientific Research of BSKY (No. XJ201211) from Anhui Medical University.

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Authors and Affiliations

  1. Institute of Clinical Pharmacology, Anhui Medical University, Hefei, 230032, China

    Peng Zhou, Xiaodan Yang, Xiaoyi Jia, Jun Yu, James Asenso, Feng Xiao, Chun Wang & Wei Wei

  2. Key Laboratory of Anti-inflammatory and Immune Medicine, Ministry of Education, Hefei, 230032, China

    Peng Zhou, Xiaodan Yang, Xiaoyi Jia, Jun Yu, James Asenso, Feng Xiao, Chun Wang & Wei Wei

  3. Anhui Collaborative Innovation Center of Anti-inflammatory and Immune Medicine, Hefei, 230032, China

    Peng Zhou, Xiaodan Yang, Xiaoyi Jia, Jun Yu, James Asenso, Feng Xiao, Chun Wang & Wei Wei

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  1. Peng Zhou
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Correspondence to Chun Wang or Wei Wei.

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Zhou, P., Yang, X., Jia, X. et al. Effect of 6′-acetylpaeoniflorin on dinitrochlorobenzene-induced allergic contact dermatitis in BALB/c mice. Immunol Res 64, 857–868 (2016). https://doi.org/10.1007/s12026-016-8788-8

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  • DOI: https://doi.org/10.1007/s12026-016-8788-8

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