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Ecto-Mesenchymal Stem Cells from Facial Process: Potential for Muscle Regeneration

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Abstract

Ecto-mesenchymal stem cells (EMSCs) originate from the cranial neural crest and participate in the formation of tooth, salivary, and muscle in early development stage. The transplantation of EMSCs, a potential source of myoblast stem cell, might improve muscle regeneration. The purpose of this study was to explore whether EMSCs have the potential to differentiate and display a myogenic phenotype in vitro the in vitro. Here, we characterized the EMSCs isolated from the facial process, and p75 + EMSCs were collected by a FACS calibur flow cytometer. In vitro, p75 + EMSCs induced by DMSO can accumulate and fuse into multinucleated myotubes and further differentiate into the skeletal muscle cells in form of cell sheet. Functional myoblast phenotypes of p75 + EMSCs were found in vivo model of muscle injury. The remarkable ability of stem cells to regenerate skeletal muscle indicated their potential role in the cell therapy and tissue engineering of the skeletal muscle.

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Acknowledgments

This work was supported by the Grants from National Natural Science Foundation of China (Grant No. 31070863, 81271097) and from the Natural Science Foundation Project of Chongqing, China (Grants No. CSTC2010BB5161 and CSTC2011BA5013).

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Authors and Affiliations

  1. Department of Stomatology, Daping Hospital & Research Institute of Surgery, Third Military Medical University, Chongqing, 400042, People’s Republic of China

    Xin Nie, Yongjun Xing, Manjin Deng, Li Gang, Rui Liu & Xiujie Wen

  2. Center for Tissue Engineering, Qin Du Stomatological College, Fourth Military Medical University, Xi’an, People’s Republic of China

    Yongjie Zhang

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  1. Xin Nie
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  2. Yongjun Xing
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  3. Manjin Deng
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  4. Li Gang
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  5. Rui Liu
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  6. Yongjie Zhang
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  7. Xiujie Wen
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Correspondence to Xiujie Wen.

Additional information

Xin Nie and Yongjun Xing have contributed equally on this study.

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Nie, X., Xing, Y., Deng, M. et al. Ecto-Mesenchymal Stem Cells from Facial Process: Potential for Muscle Regeneration. Cell Biochem Biophys 70, 615–622 (2014). https://doi.org/10.1007/s12013-014-9964-x

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  • DOI: https://doi.org/10.1007/s12013-014-9964-x

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