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Aluminium and Acrylamide Disrupt Cerebellum Redox States, Cholinergic Function and Membrane-Bound ATPase in Adult Rats and Their Offspring

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Abstract

Accumulation of aluminium and acrylamide in food is a major source of human exposure. Their adverse effects are well documented, but there is no information about the health problems arising from their combined exposure. The aim of the present study was to examine the possible neurotoxic effects after co-exposure of pregnant and lactating rats to aluminium and acrylamide in order to evaluate redox state, cholinergic function and membrane-bound ATPases in the cerebellum of adult rats and their progeny. Pregnant female rats have received aluminium (50 mg/kg body weight) via drinking water and acrylamide (20 mg/kg body weight) by gavage, either individually or in combination from the 14th day of pregnancy until day 14 after delivery. Exposure to these toxicants provoked an increase in malondialdehyde (MDA) and advanced oxidation protein product (AOPP) levels and a decrease in SOD, CAT, GPx, Na+K+-ATPase, Mg2+-ATPase and AChE activities in the cerebellum of mothers and their suckling pups. A reduction in GSH, NPSH and vitamin C levels was also observed. These changes were confirmed by histological results. Interestingly, co-exposure to these toxicants exhibited synergism based on physical and biochemical variables in the cerebellum of mothers and their progeny.

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Abbreviations

AChE:

Acetylcholinesterase

ACR:

Acrylamide

AlCl3 :

Aluminium chloride

AOPP:

Advanced oxidation protein product

CAT:

Catalase

DNPH:

Dinitrophenyl hydrazine

DTNB:

5,5′-Dithiobis-2-nitrobenzoic acid

EGL:

External granular layer

GPx:

Glutathione peroxidase

GSH:

Reduced glutathione

IGL:

Internal granular layer

MDA:

Malondialdehyde

ML:

Molecular layer

NPSH:

Non-protein thiol

PCL:

Purkinje cell layer

SOD:

Superoxide dismutase

TBA:

Thiobarbituric acid

TBARS:

Thiobarbituric acid-reactive substances;

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Acknowledgments

This work was supported by the Ministry of Higher Education and Scientific Research in Tunisia (DGRST grants, LR/11ES-53).The authors are indebted to Mr. Menaa Assili and Mr. Chedli Tmar for their skilful technical assistance.

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Authors and Affiliations

  1. Animal Physiology Laboratory, LR 11/ ES-53 Sciences Faculty of Sfax, University of Sfax, BP 1171, 3000, Sfax, Tunisia

    Imen Ghorbel, Ibtissem Ben Amara, Awatef Elwej & Najiba Zeghal

  2. Laboratoire de Génie Enzymatique et de Microbiologie, Université de Sfax-Ecole Nationale d’Ingénieurs de Sfax, 3038, Sfax, Tunisia

    Naourez Ktari

  3. Anatomopathology Laboratory, CHU Habib Bourguiba, University of Sfax, 3029, Sfax, Tunisia

    Ons Boudawara & Tahia Boudawara

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  1. Imen Ghorbel
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  2. Ibtissem Ben Amara
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  4. Awatef Elwej
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  5. Ons Boudawara
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Correspondence to Najiba Zeghal.

Ethics declarations

The general guidelines for the use and care of living animals in scientific investigations were followed (Council of European Communities: JO 86/609/CEE, 1986). The handling of the animals and the care and use of laboratory animals were approved by the Tunisian Ethical Committee of Sciences Faculty of Sfax.

Conflict of Interest Statement

The authors declare that they have no competing interests.

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Ibtissem Ben Amara and Naourez Ktari contributed equally to this work

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Ghorbel, I., Amara, I.B., Ktari, N. et al. Aluminium and Acrylamide Disrupt Cerebellum Redox States, Cholinergic Function and Membrane-Bound ATPase in Adult Rats and Their Offspring. Biol Trace Elem Res 174, 335–346 (2016). https://doi.org/10.1007/s12011-016-0716-1

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