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In Vitro Effect of Copper Chloride Exposure on Reactive Oxygen Species Generation and Respiratory Chain Complex Activities of Mitochondria Isolated from Broiler Liver

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Abstract

This study is to examine if Cu2+ can act directly on mitochondria or indirectly by producing reactive oxygen species (ROS), isolated broiler hepatic mitochondria were exposed to different concentrations of Cu2+ (10, 30, 50 μM). Respiratory chain complex activities, ROS generation, respiratory control ratio (RCR) and mitochondrial membrane potential were investigated. Dose-dependent inhibition of respiratory chain complexes and induction of ROS were observed, which coincided with decreasing RCR both with glutamate + malate or succinate. Further investigation indicated that the membrane potential determined by rhodamine 123 release decreased after CuCl2 exposure at 30 and 50 μM. In addition, the effects of the antioxidants NAC (200 μM) and GSH (200 μM) were studied at 50 μM Cu2+. The results indicate that Cu can induce mitochondrial dysfunction in excessive dose and the effect of Cu2+ exposure on respiratory chain is not site-specific, and antioxidants can protect the mitochondrial function by reducing the formation of free radicals.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (grant NO. 30871900).

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Authors and Affiliations

  1. College of Veterinary Medicine, South China Agricultural University, Guangzhou, 510642, China

    Rongsheng Su, Shining Guo, Jiaqiang Pan, Chengmei Li, Dayou Shi & Zhaoxin Tang

  2. College of Ying Dong Bioengineering, Shaoguan University, Shaoguan, 510042, China

    Rongmei Wang

  3. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang, 330045, China

    Huabin Cao

Authors
  1. Rongsheng Su
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  2. Rongmei Wang
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  3. Shining Guo
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  4. Huabin Cao
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  5. Jiaqiang Pan
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  6. Chengmei Li
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  7. Dayou Shi
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  8. Zhaoxin Tang
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Corresponding author

Correspondence to Zhaoxin Tang.

Additional information

Rongsheng Su, Rongmei Wang, Shining Guo and Huabin Cao contributed equally to this work.

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Su, R., Wang, R., Guo, S. et al. In Vitro Effect of Copper Chloride Exposure on Reactive Oxygen Species Generation and Respiratory Chain Complex Activities of Mitochondria Isolated from Broiler Liver. Biol Trace Elem Res 144, 668–677 (2011). https://doi.org/10.1007/s12011-011-9039-4

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  • DOI: https://doi.org/10.1007/s12011-011-9039-4

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