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Phospholipase A1-Catalysed Synthesis of Docosahexaenoic Acid-Enriched Phosphatidylcholine in Reverse Micelles System

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Abstract

Phosphatidylcholine enriched with docosahexaenoic acid (DHA) was successfully produced by phospholipase A1-catalysed acidolysis. Reverse micelles were firstly selected as the reaction system to avoid the process of immobilization and to ensure the efficient catalysis of phospholipase A1. Parameters were optimized for a high incorporation of phosphatidylcholine enriched with DHA (DHA-PC). A response-surface design with four factors, including the water content, enzyme loading, pH and substrate-mass ratio were used to evaluate the influence of the major factors and to predict the optimal reaction conditions. The results indicated that the optimal reaction conditions for the production of DHA-PC were a water content of 0.4%, an enzyme loading of 40%, pH 6.92 and a substrate-mass ratio of 2.13. Under these optimized conditions, 20.90% of DHA content in DHA-PC was obtained.

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Acknowledgements

This study was supported by the International Cooperation Project (grant no. 2014DFA61040), the Youth Innovation Promotion Association CAS, and the Hi-Tech Research and Development Program (863) of China (grant no. 2014ARA021701).

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  1. Tianjin Key Laboratory for Industrial Biological Systems and Bioprocess Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of SciencesTianjin, No. 32, Xiqi Road, Tianjin Airport Economic Park, Tianjin, 300308, People’s Republic of China

    Wuxi Chen, Wei Guo, Feng Gao, Limei Chen, Shulin Chen & Demao Li

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  1. Wuxi Chen
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  2. Wei Guo
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  3. Feng Gao
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Correspondence to Demao Li.

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Chen, W., Guo, W., Gao, F. et al. Phospholipase A1-Catalysed Synthesis of Docosahexaenoic Acid-Enriched Phosphatidylcholine in Reverse Micelles System. Appl Biochem Biotechnol 182, 1037–1052 (2017). https://doi.org/10.1007/s12010-016-2379-y

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