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Proteomic Profiling and the Predicted Interactome of Host Proteins in Compatible and Incompatible Interactions Between Soybean and Fusarium virguliforme

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Abstract

Sudden death syndrome (SDS) is a complex of two diseases of soybean (Glycine max), caused by the soil borne pathogenic fungus Fusarium virguliforme. The root rot and leaf scorch diseases both result in significant yield losses worldwide. Partial SDS resistance has been demonstrated in multiple soybean cultivars. This study aimed to highlight proteomic changes in soybean roots by identifying proteins which are differentially expressed in near isogenic lines (NILs) contrasting at the Rhg1/Rfs2 locus for partial resistance or susceptibility to SDS. Two-dimensional gel electrophoresis resolved approximately 1000 spots on each gel; 12 spots with a significant (P < 0.05) difference in abundance of 1.5-fold or more were picked, trypsin-digested, and analyzed using quadruple time-of-flight tandem mass spectrometry. Several spots contained more than one protein, so that 18 distinct proteins were identified overall. A functional analysis performed to categorize the proteins depicted that the major pathways altered by fungal infection include disease resistance, stress tolerance, and metabolism. This is the first report which identifies proteins whose abundances are altered in response to fungal infection leading to SDS. The results provide valuable information about SDS resistance in soybean plants, and plant partial resistance responses in general. More importantly, several of the identified proteins could be good candidates for the development of SDS-resistant soybean plants.

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Acknowledgments

The authors gratefully acknowledge the support of the Southern Illinois University, Carbondale, College of Agricultural Sciences, and Office of the Vice Chancellor for Research. Funds were provided by the United Soybean Board under the project “Application of Biotechnology to the Control of Sudden Death Syndrome (SDS)” to M. Javed Iqbal and David A. Lightfoot.

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Authors and Affiliations

  1. Department of Plant Sciences, University of California, Davis, California, 95616, USA

    M. Javed Iqbal

  2. Department of Biology, SBA School of Science and Engineering, Lahore University of Management Sciences, Lahore, 54792, Pakistan

    Maryam Majeed, Maheen Humayun & Ahmed J. Afzal

  3. Department of Molecular Biology, Microbiology, and Biochemistry, Genomics Core Facility and Center for Excellence in Soybean Research, Teaching, and Outreach, and Department of Plant Biology, Southern Illinois University, Carbondale, Illinois, 62901, USA

    David A. Lightfoot & Ahmed J. Afzal

  4. Department of Biological Sciences, Columbia University, New York, NY, 10027, USA

    Maryam Majeed

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  1. M. Javed Iqbal
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  2. Maryam Majeed
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  4. David A. Lightfoot
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Correspondence to Ahmed J. Afzal.

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M. Javed Iqbal and Maryam Majeed are joint first authors

Electronic supplementary material

Supplementary Figure 1.

Pedigree for the origins of soybean cyst nematode and sudden death syndrome resistance in EF38, showing period of release and percent heterozygosis plus heterogeneity where known (blue number). (GIF 211 kb)

High resolution image (TIFF 1521 kb)

Supplementary Figure 2.

Accession IDs of all spots in the 2D predicted interactome in Fig. 6. (PDF 169 kb)

Supplementary File 1.

Accession IDs, names, and direct interacting proteins of all the nodes represented in the interactome in Fig. 6 (separately attached, Excel file) (XLSX 16 kb)

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Iqbal, M.J., Majeed, M., Humayun, M. et al. Proteomic Profiling and the Predicted Interactome of Host Proteins in Compatible and Incompatible Interactions Between Soybean and Fusarium virguliforme . Appl Biochem Biotechnol 180, 1657–1674 (2016). https://doi.org/10.1007/s12010-016-2194-5

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