Abstract
A novel, poly(ethyl ethylene ether) inhibitor to trypsin was purified from marine cyanobacteria, Lyngbya confervoides from the coastal areas of Thalassery, North Kerala. The kinetics and the thermodynamic parameters of its interactions with the enzyme were also studied. It was demonstrated that the substrate binding, catalytic triad of the enzyme could be blocked by the inhibitor, as expressed by molecular simulation studies. The study also showed that the cyanobacterial group could prove to be a potential source of novel enzyme inhibitors for various applications.
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Acknowledgment
Funding was provided by the Ministry of Earth Science, Government of India at the Department of Biotechnology and Microbiology, Kannur University. The authors acknowledge Institute for Sophisticated Analytical Instrument Facility, KOCHI for NMR facility, and Inter University Center for Instrumentation for LC-MS at Mahatma Gandhi University; Kottayam are acknowledged for data.
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Research Highlights
• A novel poly(ethyl ethylene ether) was isolated from the marine cyanobacteria, Lyngbya confervoides
• The structure of the compound was deduced by various techniques like IR, LC MS, and NMR spectroscopies.
• Mass of the compound was found to be 948.
• The compound had a profound trypsin inhibition whose interaction was studied by isothermal titration calorimetry, molecular docking, and MD simulations.
• The ligand adopted a Kunitz-type, active-site-directed binding conformation for inhibiting the enzyme trypsin.
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Devi, A., Prasanth, S., Murugesh, E. et al. A Novel, Poly(Ethyl Ethylene Ether) Inhibitor to Trypsin from Marine Cyanobacteria, Lyngbya confervoides . Appl Biochem Biotechnol 178, 891–899 (2016). https://doi.org/10.1007/s12010-015-1916-4
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DOI: https://doi.org/10.1007/s12010-015-1916-4