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Lipid Accumulation by Pelletized Culture of Mucor circinelloides on Corn Stover Hydrolysate

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Abstract

Microbial oil accumulated by fungal cells is a potential feedstock for biodiesel production, and lignocellulosic materials can serve as the carbon source to support the fungal growth. The dilute acid pretreatment of corn stover can effectively break down its lignin structure, and this process generates a hydrolysate containing mostly xylose at very dilute concentration and numerous by-products that may significantly inhibit the cell growth. This study utilized corn stover hydrolysate as the culture media for the growth of Mucor circinelloides. The results showed that Mucor cells formed pellets during the cell growth, which facilitates the cell harvest from dilute solution. The results also showed that the inhibitory effect of furfural, 5-hydroxymethylfurfural (HMF), and acetic acid could be avoided if their concentration was low. In fact, all these by-products may be assimilated as carbon sources for the fungal growth. The results proved the feasibility to reuse the cultural broth water for acid pretreatment and then use for subsequent cell cultivation. The results will have a direct impact on the overall water usage of the process.

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References

  1. Aden, A., Ruth, M., Ibsen, K., Jechura, J., Neeves, K., Sheehan, J., et al. (2002). Lignocellulosic biomass to ethanol process design and economics utilizing co-current dilute acid prehydrolysis and enzymatic hydrolysis for corn stover. National Renewable Energy Laboratory, Golden, CO, NREL/TP-510-32438.

  2. Aggelis, G., Komaitis, M., Papanikolaou, S., & Papadopoulos, G. (1995). A mathematical model for the study of lipid accumulation in oleaginous microorganisms. 2. Study of cellular lipids of Mucor circinelloides during growth on a vegetable oil. Grasas Y Aceites, 46, 245–250.

    Article  CAS  Google Scholar 

  3. Bae, J. T., Sinha, J., Park, J. P., Song, C. H., & Yun, J. W. (2000). Optimization of submerged culture conditions for exo-biopolymer production by Paecilomyces japonica. Journal of Microbiology and Biotechnology, 10, 482–487.

    CAS  Google Scholar 

  4. Banerjee, N., Bhatnagar, R., & Viswanathan, L. (1981). Inhibition of glycolysis by furfural in Saccharomyces-Cerevisiae. European Journal of Applied Microbiology and Biotechnology, 11, 226–228.

    Article  CAS  Google Scholar 

  5. Blackman, E. D., & van Walsum, G. P. (2009). Production of renewable bioproducts and reduction of phosphate pollution through the lime pretreatment and acidogenic digestion of dairy manure. Environmental Progress & Sustainable Energy, 28, 121–133.

    Article  CAS  Google Scholar 

  6. Chen, X., Li, Z., Zhang, X., Hu, F., Ryu, D. D. Y., & Bao, J. (2009). Screening of oleaginous yeast strains tolerant to lignocellulose degradation compounds. Applied Biochemistry and Biotechnology, 159, 591–604.

    Article  CAS  Google Scholar 

  7. Dien, B. S., Cotta, M. A., & Jeffries, T. W. (2003). Bacteria engineered for fuel ethanol production: current status. Applied Microbiology and Biotechnology, 63, 258–266.

    Article  CAS  Google Scholar 

  8. Fakas, S., Papanikolaou, S., Batsos, A., Galiotou-Panayotou, M., Mallouchos, A., & Aggelis, G. (2009). Evaluating renewable carbon sources as substrates for single cell oil production by Cunninghamella echinulata and Mortierella isabellina. Biomass & Bioenergy, 33, 573–580.

    Article  CAS  Google Scholar 

  9. Farkas, V., Felinger, A., Hegedusova, A., Dekany, I., & Pernyeszi, T. (2013). Comparative study of the kinetics and equilibrium of phenol biosorption on immobilized white-rot fungus Phanerochaete chrysosporium from aqueous solution. Colloid Surf. B-Biointerfaces, 103, 381–390.

    Article  CAS  Google Scholar 

  10. Hendriks, A. T. W. M., & Zeeman, G. (2009). Pretreatments to enhance the digestibility of lignocellulosic biomass. Bioresource Technology, 100, 10–18.

    Article  CAS  Google Scholar 

  11. Heredia-Arroyo, T., Wei, W., & Hu, B. (2010). Oil accumulation via heterotrophic/mixotrophic Chlorella protothecoides. Applied Biochemistry and Biotechnology, 162, 1978–1995.

    Article  CAS  Google Scholar 

  12. Hu, C., Zhao, X., Zhao, J., Wu, S., & Zhao, Z. K. (2009). Effects of biomass hydrolysis by-products on oleaginous yeast Rhodosporidium toruloides. Bioresource Technology, 100, 4843–4847.

    Article  CAS  Google Scholar 

  13. Huang, C., Zong, M.-H., Wu, H., & Liu, Q.-P. (2009). Microbial oil production from rice straw hydrolysate by Trichosporon fermentans. Bioresource Technology, 100, 4535–4538.

    Article  CAS  Google Scholar 

  14. Jackson, F. M., Fraser, T. C. M., Smith, M. A., Lazarus, C., Stobart, A. K., & Griffiths, G. (1998). Biosynthesis of C-18 polyunsaturated fatty acids in microsomal membrane preparations from the filamentous fungus Mucor circinelloides. European Journal of Biochemistry, 252, 513–519.

    Article  CAS  Google Scholar 

  15. Lee, R. F., Hagen, W., & Kattner, G. (2006). Lipid storage in marine zooplankton. Marine Ecology-Progress Series, 307, 273–306.

    Article  CAS  Google Scholar 

  16. Lin, Y., & Tanaka, S. (2006). Ethanol fermentation from biomass resources: current state and prospects. Applied Microbiology and Biotechnology, 69, 627–642.

    Article  CAS  Google Scholar 

  17. McIntyre, M., Breum, J., Arnau, J., & Nielsen, J. (2002). Growth physiology and dimorphism of Mucor circinelloides (syn. racemosus) during submerged batch cultivation. Applied Microbiology and Biotechnology, 58, 495–502.

    Article  CAS  Google Scholar 

  18. Miao, X., & Wu, Q. (2006). Biodiesel production from heterotrophic microalgal oil. Bioresource Technology, 97, 841–846.

    Article  CAS  Google Scholar 

  19. Millati, R., Karimi, K., Edebo, L., Niklasson, C., & Taherzadeh, M. J. (2008). Ethanol production from xylose and wood hydrolyzate by Mucor indicus at different aeration rates. Bioresources, 3, 1020–1029.

    CAS  Google Scholar 

  20. Ohgren, K., Bura, R., Saddler, J., & Zacchi, G. (2007). Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover. Bioresource Technology, 98, 2503–2510.

    Article  Google Scholar 

  21. Palmqvist, E., & Hahn-Hagerdal, B. (2000). Fermentation of lignocellulosic hydrolysates. II: inhibitors and mechanisms of inhibition. Bioresource Technology, 74, 25–33.

    Article  CAS  Google Scholar 

  22. Pampulha, M. E., & Loureirodias, M. C. (1989). Combined effect of acetic-acid, pH and ethanol on intracellular pH of fermenting yeast. Applied Microbiology and Biotechnology, 31, 547–550.

    Article  CAS  Google Scholar 

  23. Ratledge, C. and Wilkinson, S. G. (1989) Microbial lipids. ed. Academic Press.

  24. Sathya, R., Pradeep, B. V., Angayarkanni, J., & Palaniswamy, M. (2009). Production of milk clotting protease by a local isolate of Mucor circinelloides under SSF using agro-industrial wastes. Biotechnology and Bioprocess Engineering, 14, 788–794.

    Article  CAS  Google Scholar 

  25. Taherzadeh, M. J., Niklasson, C., & Liden, G. (1997). Acetic acid—friend or foe in anaerobic batch conversion of glucose to ethanol by Saccharomyces cerevisiae? Chemical Engineering Science, 52, 2653–2659.

    Article  CAS  Google Scholar 

  26. Talebnia, F., Karakashev, D., & Angelidaki, I. (2010). Production of bioethanol from wheat straw: an overview on pretreatment, hydrolysis and fermentation. Bioresource Technology, 101, 4744–4753.

    Article  CAS  Google Scholar 

  27. Vicente, G., Bautista, L. F., Rodriguez, R., Gutierrez, F. J., Sadaba, I., Ruiz-Vazquez, R. M., et al. (2009). Biodiesel production from biomass of an oleaginous fungus. Biochemical Engineering Journal, 48, 22–27.

    Article  CAS  Google Scholar 

  28. Wang, M., Wang, J., & Tan, J. X. (2011). Lignocellulosic bioethanol: status and prospects. Energy Sources Part a—Recovery Utilization and Environmental Effects, 33, 612–619.

    Article  CAS  Google Scholar 

  29. Wyman, C. E., Dale, B. E., Elander, R. T., Holtzapple, M., Ladisch, M. R., & Lee, Y. Y. (2005). Coordinated development of leading biomass pretreatment technologies. Bioresource Technology, 96, 1959–1966.

    Article  CAS  Google Scholar 

  30. Xia, C., Zhang, J., Zhang, W., & Hu, B. (2011). A new cultivation method for microbial oil production: cell pelletization and lipid accumulation by Mucor circinelloides. Biotechnology for Biofuels, 4, 15.

    Article  Google Scholar 

  31. Xiao, J. H., Chen, D. X., Liu, J. W., Liu, Z. L., Wan, W. H., Fang, N., et al. (2004). Optimization of submerged culture requirements for the production of mycelial growth and exopolysaccharide by Cordyceps jiangxiensis JXPJ 0109. Journal of Applied Microbiology, 96, 1105–1116.

    Article  CAS  Google Scholar 

  32. Yang, B., & Wyman, C. E. (2004). Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnology and Bioengineering, 86, 88–95.

    Article  CAS  Google Scholar 

  33. Zhang, J. G., & Hu, B. (2012). A novel method to harvest microalgae via co-culture of filamentous fungi to form cell pellets. Bioresource Technology, 114, 529–535.

    Article  CAS  Google Scholar 

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Acknowledgments

The research is supported by the UMN Grand-in-Aid program.

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Authors and Affiliations

  1. Department of Bioproducts and Biosystems Engineering, University of Minnesota, 316 BAE, 1390 Eckles Ave, Saint Paul, MN, 55108-6005, USA

    Cristiano E. R. Reis & Bo Hu

  2. School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China

    Jianguo Zhang

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  1. Cristiano E. R. Reis
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Correspondence to Bo Hu.

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Reis, C.E.R., Zhang, J. & Hu, B. Lipid Accumulation by Pelletized Culture of Mucor circinelloides on Corn Stover Hydrolysate. Appl Biochem Biotechnol 174, 411–423 (2014). https://doi.org/10.1007/s12010-014-1112-y

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  • DOI: https://doi.org/10.1007/s12010-014-1112-y

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