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Isolation, Purification and Characterisation of Low Molecular Weight Xylanase from Bacillus pumilus SSP-34

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Abstract

Low molecular weight endo-xylanase from Bacillus pumilus SSP-34 was purified to homogeneity using ion exchange and size exclusion chromatographies. Xylanases were isolated by novel purification protocol which includes the use of anion exchange matrix such as DEAE Sepharose CL 6B with less affinity towards enzyme protein. The purified B. pumilus SSP-34 have a molecular weight of 20 kDa, with optimum pH and temperature at 6.0 and 50 °C, respectively. The enzyme was stable at 50 °C for 30 min. It showed remarkable stability at pH values ranging from 4.5 to 9 when the reaction was carried out at 50 °C. K m and V max values, determined with oats spelts xylan were 6.5 mg ml−1 and 1,233 μmol min−1 mg−1 protein, respectively, and the specific activity was 1,723 U mg−1

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Acknowledgements

I am very grateful to my late research guide, Dr. P. Prema, who worked as a senior scientist in Biotechnology Division, National Institute for Interdisciplinary Sciences and Technology (Formerly Regional Research Laboratory), Thiruvananthapuram 695019. Without her help, this work would not have been possible. I am also thankful to NIIST Trivandrum and CSIR New Delhi, INDIA. I use this opportunity to express my gratitude to the Director, Department of Collegiate Education, Government of Kerala, India, and the Principal, University College, Trivandrum.

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  1. Department of Botany and Research Centre, University College, Thiruvananthapuram, 695034, Kerala, India

    S. Subramaniyan

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Subramaniyan, S. Isolation, Purification and Characterisation of Low Molecular Weight Xylanase from Bacillus pumilus SSP-34. Appl Biochem Biotechnol 166, 1831–1842 (2012). https://doi.org/10.1007/s12010-012-9600-4

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