Skip to main content
SpringerLink
Log in

High activity xylanase from thermotolerantStreptomyces T7: Cultural conditions and enzyme properties

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Summary

A thermotolerantStreptomyces T7 produced 70–72 U/ml of extracellular xylanase activity when grown at 50°C in submerged culture, in à medium containing 5% wheat bran as a carbon source. Among the various sugars tested, maltose showed the highest activity of 8 U/ml. Pure xylan was less effective as an inducer as compared to wheat bran. Ammonium sulphate at a concentration of 0.7% was found to be optimum for maximum yield of the enzyme. The optimum period and pH for maximum production were 72th and 7.0, respectively. The culture filtrate was devoid of amylase, cellulase and B-xylosidase activity. The xylanase was exceptionally stable and did not show any loss in activity after storage at 50°C at pH 5.0 for 6 days.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ball, A. S. and McCarthy, A. J. (1989)J. Appl. Bacteriol 66, 439–444.

    Google Scholar 

  • Holtz, C., Kaspari, H. and Klemme, J-H (1991)Antonie Van Leeuwenhoek 59, 1–7.

    Google Scholar 

  • Ishaque, M. and Kluepfel, D. (1981)Biotechnol Lett. 3, 481–486.

    Google Scholar 

  • Keskar, S. S., Rao, M. B. and Deshpande, V. V. (1992)Biochem. J. 281, 601–605.

    Google Scholar 

  • Keskar, S. S., Srinivasan, M. C. and Deshpande, V. V. (1989),Biochem. J. 261, 49–55.

    Google Scholar 

  • Kluepfel, D., Shareck, F., Mondou, F. and Morosoli, R. (1986)Appl. Microbiol. Biotechnol 24, 230–234.

    Google Scholar 

  • Mackenzie, C. R., Bilous, D., Schneider, H. and Johnson, K. G. (1987)Appl. Env. Microbiol 53, 2835–2839.

    Google Scholar 

  • Mandels, M. and Weber, J. (1969)Adv. Chem. Ser. 95, 395–414.

    Google Scholar 

  • McCarthy, A. J. (1987)FEMS Microbiol Rev 46, 145–163.

    Google Scholar 

  • McCarthy, A. J., Peace, E. and Broda, P. (1985)Appl. Microbiol. Biotechnol 21, 238–244.

    Google Scholar 

  • Paice, M. G. and Jurasek, L. (1984)J. Wood Chem. Technol. 4, 187–198.

    Google Scholar 

  • Pilon, L. Barbe, M. C., Desrochers, M., Jurasek, L. and Neumann, P. J. (1982)Biotechnol. Bioeng. 24, 2063–2076.

    Google Scholar 

  • Srinivasan, M. C. Vartak, H. G., Powar, V. K., Rele, M. V., and Bastawde, K. B. (1984)Biotechnol Lett 6, 715–718.

    Google Scholar 

  • Vyas, P., Chauthaiwale, V., Phadatare, S., Deshpande, V. and Srinivasan, M. C. (1990)Biotechnol. Lett. 12, 225–228.

    Google Scholar 

  • Zimmermann, W., Winter, B. and Broda, P. (1988)FEMS Microbiol Lett. 55, 181–186.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Division of Biochemical Sciences, National Chemical Laboratory, 411 008, Pune, India

    Sulabha S. Keskar

Authors
  1. Sulabha S. Keskar
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Keskar, S.S. High activity xylanase from thermotolerantStreptomyces T7: Cultural conditions and enzyme properties. Biotechnol Lett 14, 481–486 (1992). https://doi.org/10.1007/BF01023171

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01023171

Keywords

Search

Navigation