Skip to main content
SpringerLink
Log in

Isolation and Characterization of Exopolysaccharides Produced by the Cyanobacterium Limnothrix redekei PUPCCC 116

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Limnothrix redekei PUPCCC 116, a filamentous cyanobacterium, has been identified through 16S rRNA gene sequencing. Exopolysaccharides (EPS) of this organism have been isolated and characterized chemically, and its rheological properties were compared with commercial xanthan. The organism produced 304 µg EPS/ml culture in 21 days. The rate of EPS production was maximum (313 µg EPS/mg protein/day) during the initial days of growth, and it decreased to 140 µg EPS/mg protein/day during 18-21 days of growth. Chemical analysis of EPS revealed the presence of glucose/mannose, ribose, rhamnose, and uronic acid. Fourier transformed infrared spectrum of EPS further revealed the presence of methyl and carboxyl groups besides C–N groups indicating the presence of peptidyl moieties. Elemental analysis of EPS showed the presence of 4.97% N. The organism under continuous light produced 102% more EPS compared to when grown under a light/dark cycle of 14/10 h. The rheological properties of EPS were comparable with commercial xanthan gum.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Wingender, J., Neu, T. R., & Flemming, H. C. (1999). In J. Wingender, T. R. Neu, & H. C. Flemming (Eds.), Microbial extracellular polymeric substances (pp. 1–19). Berlin: Springer.

    Google Scholar 

  2. Trabelsi, L., M’sakni, N. H., Ouada, H. B., Bacha, H., & Roudesli, S. (2009). Biotechnology and Bioprocess Engineering, 14, 27–31.

    Article  CAS  Google Scholar 

  3. Parikh, A., & Madamwar, D. (2006). Bioresource Technology, 97, 1822–1827.

    Article  CAS  Google Scholar 

  4. Zou, X., Sun, M., & Guo, X. (2006). International Journal of Biodeterioration and Biodegradation, 58, 89–93.

    Article  CAS  Google Scholar 

  5. Chi, Z., Su, C. D., & Lu, W. D. (2007). Bioresource Technology, 98, 1329–1332.

    Article  CAS  Google Scholar 

  6. Duan, X., Chi, Z., Wang, L., & Wang, X. (2008). Carbohydrate Polymers, 73, 587–593.

    Article  CAS  Google Scholar 

  7. Selbmann, L., Onofri, S., Fenice, M., Federici, F., & Petruccioli, M. (2002). Research in Microbiology, 153, 585–592.

    Article  CAS  Google Scholar 

  8. De Philippis, R., & Vincenzini, M. (1998). FEMS Microbiology Reviews, 22, 151–175.

    Article  Google Scholar 

  9. De Philippis, R., Sili, C., Paperi, R., & Vincenzini, M. (2001). Journal of Applied Phycology, 13, 293–299.

    Article  Google Scholar 

  10. Ozturk, S., Aslim, B. (2009). Environmental Science and Pollution Research. doi:10:1007/S11356-009-0233-2.

  11. Freire-Nordi, C. S., Vieira, A. A. H., & Nascimento, O. R. (2005). Process Biochemistry, 40, 2215–2224.

    Article  CAS  Google Scholar 

  12. Ozturk, S., Aslim, B., & Suludere, Z. (2009). Bioresource Technology, 100, 5588–5593.

    Article  CAS  Google Scholar 

  13. Bender, J., & Phillips, P. (2004). Bioresource Technology, 94, 229–238.

    Article  CAS  Google Scholar 

  14. Ozturk, S., & Aslim, B. (2008). Environmental Science and Pollution Research, 15, 478–480.

    Article  CAS  Google Scholar 

  15. Sutherland, I. W. (1998). Trends in Biotechnology, 16, 41–46.

    Article  CAS  Google Scholar 

  16. Safferman, R. S., & Morris, M. E. (1964). Journal of Bacteriology, 88, 771–775.

    CAS  Google Scholar 

  17. Nubel, U., Garcia-Pichel, F., & Muyzer, G. (1997). Applied and Environmental Microbiology, 63, 3327–3332.

    CAS  Google Scholar 

  18. Reddy, K. J., Soper, B. W., Tang, J., & Bradley, R. L. (1996). World Journal of Microbiology and Biotechnology, 12, 311–318.

    Article  Google Scholar 

  19. Seifter, S., Dayton, S., Novic, B., & Muntwyler, E. (1950). Archives of Biochemistry, 25, 191–200.

    CAS  Google Scholar 

  20. Chaplin, M. F. (1986). In M. F. Chaplin & J. F. Kennedy (Eds.), Carbohydrate analysis: A practical approach (pp. 1–36). Oxford: IRL.

    Google Scholar 

  21. Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Journal of Biological Chemistry, 193, 265–275.

    CAS  Google Scholar 

  22. Kumar, A. S., Mody, K., & Jha, B. (2007). Journal of Basic Microbiology, 47, 103–117.

    Article  CAS  Google Scholar 

  23. Mishra, A., & Jha, B. (2009). Bioresource Technology, 100, 3382–3386.

    Article  CAS  Google Scholar 

  24. Kumar, C. G., Joo, H., Kavali, R., Choi, J., & Chang, C. (2004). World Journal of Microbiology and Biotechnology, 20, 833–836.

    Article  Google Scholar 

  25. Shah, V., Garg, N., & Madamwar, D. (1999). Applied Biochemistry and Biotechnology, 82, 81–90.

    Article  CAS  Google Scholar 

  26. De Philippis, R., Sili, C., Tassinato, G., Vincenzini, M., & Materassi, R. (1991). Bioresource Technology, 38, 101–104.

    Article  Google Scholar 

  27. Staats, N., Stal, L. J., & Mur, L. R. (2000). Journal of Experimental Marine Biology and Ecology, 249, 13–27.

    Article  CAS  Google Scholar 

  28. Giroldo, D., & Vieira, A. A. H. (2002). Journal of Applied Phycology, 14, 185–191.

    Article  CAS  Google Scholar 

  29. Lupi, F. M., Fernandes, H. M. L., Tome, M. M., Sa-Correia, I., & Novais, J. M. (1994). Enzyme and Microbial Technology, 16, 546–550.

    Article  CAS  Google Scholar 

  30. Otero, A., & Vincenzini, M. (2003). Journal of Biotechnology, 102, 143–152.

    Article  CAS  Google Scholar 

  31. Trabelsi, L., Ouada, H. B., Bacha, H., & Ghoul, M. (2009). Journal of Applied Phycology, 21, 405–412.

    Article  CAS  Google Scholar 

  32. De Philippis, R., Margheri, M. C., Pelosi, E., & Ventura, S. (1993). Journal of Applied Phycology, 5, 387–394.

    Article  Google Scholar 

  33. Hu, C., Liu, Y., Paulsen, B. S., Petersen, D., & Klavenses, D. (2003). Carbohydrate Polymers, 54, 33–42.

    Article  CAS  Google Scholar 

  34. Sudo, H., Grant Burgess, J., Takemasa, H., Nakamura, N., & Matsunaga, T. (1995). Current Microbiology, 30, 219–222.

    Article  CAS  Google Scholar 

  35. De Philippis, R., Ena, A., Paperi, R., Sili, C., & Vincenzini, M. (2000). Journal of Applied Phycology, 12, 401–407.

    Article  Google Scholar 

  36. Vicente-Garcia, V., Rios-Leal, E., Calderon-Dominguez, G., Canizares-Villanueva, R. O., & Olvera-Ramirez, R. (2004). Biotechnology and Bioengineering, 85, 306–310.

    Article  CAS  Google Scholar 

  37. Sutherland, I. W. (1994). Biotechnological Advances, 12, 393–448.

    Article  CAS  Google Scholar 

  38. Shepherd, R., Rockey, J., Sutherland, I. W., & Roller, S. (1995). Journal of Biotechnology, 40, 207–217.

    Article  Google Scholar 

  39. Enriquez, L. G., Hwang, J. W., Hong, G. P., Bati, N. A., & Flick, G. J. (1989). In G. Charalambons & G. Doxastakis (Eds.), Plant and microbial food gums (pp. 335–416). Amsterdam: Elsevier.

    Google Scholar 

  40. Wang, Y., Wang, L. J., Li, D., Xue, J., & Mao, Z. H. (2009). Carbohydrate Polymers, 78, 213–219.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors are thankful to the Head & Coordinator DRS and ASIST, Department of Botany, Punjabi University, Patiala and the Director, IHBT, Palampur for laboratory facilities. Financial assistance from the Council of Scientific & Industrial Research, New Delhi with Research Scheme no. 38(1189)/08/EMR-II is also acknowledged.

Author information

Authors and Affiliations

  1. Department of Botany, Punjabi University, Patiala, 147 002, India

    J. I. S. Khattar, D. P. Singh, Namita Jindal & Y. Singh

  2. Plant Pathology and Microbiology, Institute of Himalayan Bioresource Technology, Palampur, 176 061, India

    N. Kaur, P. Rahi & A. Gulati

Authors
  1. J. I. S. Khattar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. P. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Namita Jindal
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. N. Kaur
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. Rahi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Gulati
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to J. I. S. Khattar.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Khattar, J.I.S., Singh, D.P., Jindal, N. et al. Isolation and Characterization of Exopolysaccharides Produced by the Cyanobacterium Limnothrix redekei PUPCCC 116. Appl Biochem Biotechnol 162, 1327–1338 (2010). https://doi.org/10.1007/s12010-010-8922-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-010-8922-3

Keywords

Search

Navigation