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Physico-Mechanical Characterization and Antimicrobial Properties of Fish Protein Isolate/Fish Skin Gelatin-Zinc Oxide (ZnO) Nanocomposite Films

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Abstract

Antimicrobial nanocomposite films based on fish protein isolate (FPI)/fish skin gelatin (FSG) (1/1 w/w) prepared at pH 3 and 11 containing zinc oxide nanoparticles (ZnONP) at different levels (0–4 % w/w of protein) were characterised. At both pH 3 and pH 11, tensile strength (TS) increased, whilst elongation at break (EAB) and water vapour permeability (WVP) decreased as ZnONP content increased (p < 0.05). FPI/FSG-ZnO nanocomposite films had lower transparency, b*-values (yellowness), and ∆E*-values (total colour difference) than the control FPI/FSG film (p < 0.05). Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopic analysis revealed that there was interaction between ZnONP and protein in the film matrix. Based on thermogravimetric analysis, ZnONP could improve the thermal stability of the nanocomposite films. X-ray diffraction (XRD) analysis confirmed the crystalline structure of the ZnONP in the nanocomposite films. FPI/FSG-ZnO nanocomposite films, especially those prepared at pH 3, exhibited strong antibacterial activity and thus could be used as an active food packaging material.

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Acknowledgments

The authors would like to express their sincere thanks to Prince of Songkla University and National Research Council of Thailand for their financial support. The TRF Senior Research Scholar programme is gratefully acknowledged.

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Authors and Affiliations

  1. Department of Food Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Yasir Ali Arfat, Soottawat Benjakul & Punnanee Sumpavapol

  2. Department of Material Product Technology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, Songkhla, 90112, Thailand

    Thummanoon Prodpran & Ponusa Songtipya

Authors
  1. Yasir Ali Arfat
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  2. Soottawat Benjakul
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  3. Thummanoon Prodpran
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  4. Punnanee Sumpavapol
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  5. Ponusa Songtipya
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Correspondence to Yasir Ali Arfat.

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Arfat, Y.A., Benjakul, S., Prodpran, T. et al. Physico-Mechanical Characterization and Antimicrobial Properties of Fish Protein Isolate/Fish Skin Gelatin-Zinc Oxide (ZnO) Nanocomposite Films. Food Bioprocess Technol 9, 101–112 (2016). https://doi.org/10.1007/s11947-015-1602-0

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