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Imaging of Intact Ovine m. semimembranosus by Confocal Raman Microscopy

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Abstract

This paper reports for the first time the application of Raman confocal microscopic imaging to determine the impact of chemical and spatial differences in Raman spectra of ovine m. semimembranosus (SM) on indicators of tenderness and the potential to classify SMs based on shear force values. Chemical images of integrated intensities were generated and analysed against traditional indicators of tenderness. Results indicated that using the tyrosine doublet at 853 cm−1, it was possible to classify SMs into categories based on tenderness and soluble collagen content (P < 0.05). A promising classification was also achieved for the classification of SMs based on sarcomere lengths (P < 0.05) using the α-helix (930 cm−1) signal. Despite integrating the intensities and reducing wavenumbers values used in the analysis, the Raman spectra were still complex and the impact of spectral overlap from multiple compounds with similar chemical composition will need to be determined by future research.

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Acknowledgments

This work has been financially supported by the Australian Meat Processor Corporation (AMPC) and Meat and Livestock Australia (MLA), as is the senior author by scholarship. The authors also acknowledge the contribution of Matt Kerr, Tracy Lamb and Kristy Bailes (NSW DPI) who assisted in the measurement of the samples.

Conflict of Interest

The authors declare they have no competing interests.

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Authors and Affiliations

  1. Centre for Sheep and Red Meat Development, NSW Department of Primary Industries, Cowra, Australia

    Stephanie M. Fowler & David L. Hopkins

  2. Graham Centre for Agricultural Innovation, NSW Department of Primary Industries and Charles Sturt University, Wagga Wagga, Australia

    Stephanie M. Fowler, Peter Wynn & David L. Hopkins

  3. Centre for Biospectroscopy, School of Chemistry, Monash University, Melbourne, Australia

    Bayden R. Wood

  4. Department of Health, Animal Science and Food Safety, University of Milan, Milan, Italy

    Matteo Ottoboni & Gianluca Baldi

  5. Orange Institute of Agriculture, NSW Department of Primary Industries, Orange, Australia

    Remy van de Ven

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  1. Stephanie M. Fowler
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  2. Bayden R. Wood
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  3. Matteo Ottoboni
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  4. Gianluca Baldi
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Correspondence to Stephanie M. Fowler.

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Fowler, S.M., Wood, B.R., Ottoboni, M. et al. Imaging of Intact Ovine m. semimembranosus by Confocal Raman Microscopy. Food Bioprocess Technol 8, 2279–2286 (2015). https://doi.org/10.1007/s11947-015-1574-0

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  • DOI: https://doi.org/10.1007/s11947-015-1574-0

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