Skip to main content
Log in

Phylloquinone (vitamin K1) and dihydrophylloquinone content of fats and oils

  • Published:
Journal of the American Oil Chemists' Society

Abstract

Assessment of vitamin K (VK) dietary intakes has been limited by the incompleteness of VK food composition data for the U.S. food supply, particularly for VK-rich oils. The phylloquinone (VK-1) and 2′,3′-dihydrophylloquinone (dK) concentrations of margarines and spreads (n=43), butter (n=4), shortening (n=4), vegetable oils (n=6), and salad dressings (n=24) were determined by RP-HPLC with fluorescence detection. Each sample represented a composite of units or packages obtained from 12 or 24 outlets, which were geographically representative of the U.S. food supply. Butter, which is derived from animal fat sources, had less VK-1 compared to vegetable oil sources. The VK-1 and dK of the margarines and spreads increased with fat content and the degree of hydrogenation, respectively. In some margarines or spreads and in all shortenings, the dK concentrations were higher than the corresponding VK-1 concentrations. As the fat content of salad dressings increased, the VK-1 concentrations also increased. Fat-free foods had <1 μg/100 g of either form of the vitamin. No dK was detected in the salad dressings or oils tested. Some margarines, spreads, and salad dressings may be significant sources of vitamin K in the U.S. food supply.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. National Academy of Science, Institute of Medicine, Dietary Reference Intakes for Vitamin A, Vitamin K, Arsenic, Boron, Chromium, Copper, Iodine, Iron, Manganese, Molybdenum, Nickel, Silicon, Vanadium, and Zinc, National Academy Press, Washington, DC, 2001.

    Google Scholar 

  2. Booth, S., J. Sadowski, J. Weihrauch, and G. Ferland, Vitamin K-1 (phylloquinone) Content of Foods: A Provisional Table, J. Food Comp. Anal. 6:109–120 (1993).

    Article  CAS  Google Scholar 

  3. United States Department of Agriculture, Agricultural Research Service, Human Nutrition Information Service, Provisional Table on the Vitamin K Content of Foods, HNIS/PT-104, Washington, DC, 1994.

  4. Bolton-Smith, C., R.J. Price, S.T. Fenton, D.J. Harrington, and M.J. Shearer, Compilation of a Provisional U.K. Database for the Phylloquinone (vitamin K1) Content of Foods, Br. J. Nutr. 83:389–399 (2000).

    CAS  Google Scholar 

  5. Deharveng, G., U.R. Charrondiere, N. Slimani, D.A. Southgate, and E. Ribolt, Comparison of Nutrients in the Food Composition Tables Available in the Nine European Countries Participating in EPIC. European Prospective Investigation into Cancer and Nutrition, Eur. J. Clin. Nutr. 53:60–79 (1999).

    Article  CAS  Google Scholar 

  6. McKeown, N.M., H.M. Rasmussen, J.M. Charnley, R.J. Wood, and S.L. Booth, Accuracy of Phylloquinone (vitamin K-1) Data in 2 Nutrient Databases as Determined by Direct Laboratory Analysis of Diets, J. Am. Diet Assoc. 100:1201–1204 (2000).

    Article  CAS  Google Scholar 

  7. Booth, S., J. Sadowski, and J. Pennington, Phylloquinone (vitamin K-1) Content of Foods in the U.S. Food and Drug Administration’s Total Diet Study, J. Agric. Food Chem. 43:1574–1579 (1995).

    Article  CAS  Google Scholar 

  8. Davidson, K., S. Booth, G. Dolnikowski, and J. Sadowski, Conversion of Vitamin K-1 to 2′,3′-Dihydrovitamin K-1 During the Hydrogenation of Vegetable Oils, 44:980–983 (1996).

    Article  CAS  Google Scholar 

  9. Pehrsson, P., D. Haytowitz, J. Holden, C. Perry, and D. Beckler, USDA’s National Food and Nutrient Analysis Program: Food Sampling, J. Food Compos. Anal. 13:379–389 (2000).

    Article  CAS  Google Scholar 

  10. Haytowitz, D.B., P.R. Pehrsson, J. Smith, S.E. Gebhardt, R.H. Matthews, and B.A. Anderson, Key Foods: Setting Priorities for Nutrient Analysis, 9:331–364 (1996).

    Article  Google Scholar 

  11. Goodall, C.R., K. Kafadar, and J.W. Tukey, Computing and Using Rural Versus Urban Measures in Statistical Applications, Am. Stat. 52:101–111 (1998).

    Article  Google Scholar 

  12. Chaudhuri, A., and J.W.E. Vos, Unified Theory and Strategies of Survey Sampling, NHC, New York, 1988.

    Google Scholar 

  13. Booth, S.L., and J.A. Sadowski, Determination of Phylloquinone in Foods by High-Performance Liquid Chromatography, Methods Enzymol. 282:446–456 (1997).

    Article  CAS  Google Scholar 

  14. Piironen, V., T. Koivu, O. Tammisalo, and P. Mattila, Determination of Phylloquinone in Oils, Margarines, and Butter by High-Performance Liquid Chromatography with Electrochemical Detection, Food Chem. 59:472–480 (1997).

    Article  Google Scholar 

  15. Cook, K., G. Mitchell, E. Grundel, and J. Rader, HPLC Analysis for trans-Vitamin K1 and Dihydro-Vitamin K1 in Margarines and Margarine-like Products Using C30 Stationary Phase, 67:79–88 (1999).

    Article  CAS  Google Scholar 

  16. Ferland, G., and J. Sadowski, Vitamin K1 (phylloquinone) Content of Edible Oils: Effects of Heating and Light Exposure, J. Agric. Food Chem. 40:1869–1873 (1992).

    Article  CAS  Google Scholar 

  17. Bolton-Smith, C., R. Price, and M. Shearer, Decreasing Phylloquinone (vitamin K1) and Total Fat Intake in a 10-Year Longitudinal Study of Scottish Adults, Proc. Nutr. Soc. 59:24A (2000) (abstract).

    Google Scholar 

  18. Koivu, T., V. Piironen, A.-M. Lampi, and P. Mattila, Dihydro-vitamin K1 in Oils and Margarines, Food Chem. 64:411–414 (1999).

    Article  CAS  Google Scholar 

  19. Booth, S., A. Lichtenstein, M. O’Brien-Morse, N. McKeown, R. Wood, E. Saltzman, and C. Gundberg, Impact of a Hydrogenated Form of Vitamin K on Bone Formation and Resorption, Am. J. Clin. Nutr. 74:783–790 (2001).

    CAS  Google Scholar 

  20. Booth, S., N. McKeown, A. Lichtenstein, M. Morse, K. Davidson, R. Wood, and C. Gundberg, A Hydrogenated Form of Vitamin K: Its Relative Bioavailability and Presence in the Food Supply, J. Food Compos. Anal. 13:311–317 (2000).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Sarah L. Booth.

About this article

Cite this article

Peterson, J.W., Muzzey, K.L., Haytowitz, D. et al. Phylloquinone (vitamin K1) and dihydrophylloquinone content of fats and oils. J Amer Oil Chem Soc 79, 641–646 (2002). https://doi.org/10.1007/s11746-002-0537-z

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11746-002-0537-z

Key Words

Navigation