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Trans10,cis15 18:2 Isolated from Beef Fat Does Not Have the Same Anti-Adipogenic Properties as Trans10,cis12–18:2 in 3T3-L1 Adipocytes

  • Original Article
  • Published:
Lipids

Abstract

During ruminal biohydrogenation of α-linolenic acid, a non-conjugated non-methylene interrupted dienoic acid is formed containing a t10 double bond, namely t10,c15–18:2. The present study was designed to examine whether t10,c15–18:2 would exert similar anti-adipogenic effects compared to t10,c12–18:2 in 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were treated with 35 or 70 µM of LNA, t10,c12–18:2, t10,c15–18:2, or bovine serum albumin (BSA) vehicle control for 120 h. Cellular triacylglycerol and protein were quantified using commercial colorimetric kits. Cells were analyzed for fatty acid composition and gene expression using gas chromatography and quantitative PCR, respectively. Trans10,cis12–18:2 decreased (P < 0.05) the adipocyte triacylglycerol (TAG) content, which was mainly related to a reduction in saturated fatty acids (SFA; e.g., 16:0 and 15:0) and cis monounsaturated fatty acids (c-MUFA; e.g., c9–16:1 and c9–18:1). Trans10,cis12 also decreased (P < 0.05) the expression of genes related to fatty acid synthesis (ACACA, FASN), delta-9 desaturation (SCD1), fatty acid elongation (ELOVL5), and fatty acid uptake (LPL) and upregulated (P < 0.05) the expression of the rate-liming enzyme involved in fatty acid β-oxidation (CPT1). In contrast, LNA and t10,c15–18:2 did not affect the gene expression and cellular content of the TAG, SFA, c-MUFA, or SCD1 indices in adipocytes. Our findings suggest that t10,c15–18:2, despite having structural similarity to t10,c12–18:2 (presence of a trans-10 double bond), does not exert anti-adipogenic effects in 3T3-L1 adipocytes.

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Abbreviations

ACACA:

Acetyl-CoA carboxylase

Ag+-HPLC:

High-performance liquid chromatography

Ag+-SPE:

Silver-ion solid phase extraction

ALA:

α-Linolenic acid

BHI:

Biohydrogenation intermediates

BSA:

Bovine serum albumin

CLA:

Conjugated linoleic acid

CPT1:

Carnitine palmitoyltransferase 1

ELOVL5:

Elongation of very long chain fatty acids protein 5

FASN:

Fatty acid synthase

LNA:

Linoleic acid

LPL:

Lipoprotein lipase

MUFA:

Monounsaturated fatty acids

PPARγ:

Peroxisome proliferator-activated receptor gamma

PUFA:

Polyunsaturated fatty acids

SCD1:

Stearoyl-CoA desaturase-1

SFA:

Saturated fatty acids

SREBF1:

Sterol regulatory element-binding factor-1

TAG:

Triacylglycerol

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Acknowledgments

Study funding was provided through AAFC-Peer Review program (Grant #13-1144). P. Vahmani acknowledges NSERC post-doctoral funding provided by the AAFC-Peer Review program. The authors thank Mr. Alec. D. Mitchell for his valuable assistance in gene expression and triglyceride analyses.

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Authors and Affiliations

  1. Lacombe Research and Development Centre, Agriculture and Agri-Food Canada, Lacombe, Alberta, Canada

    Payam Vahmani, William J. Meadus, David C. Rolland, Pascale Duff & Michael E. R. Dugan

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  1. Payam Vahmani
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  2. William J. Meadus
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Correspondence to Michael E. R. Dugan.

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Vahmani, P., Meadus, W.J., Rolland, D.C. et al. Trans10,cis15 18:2 Isolated from Beef Fat Does Not Have the Same Anti-Adipogenic Properties as Trans10,cis12–18:2 in 3T3-L1 Adipocytes. Lipids 51, 1231–1239 (2016). https://doi.org/10.1007/s11745-016-4192-3

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  • DOI: https://doi.org/10.1007/s11745-016-4192-3

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