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PAM, OLA, and LNA are Differentially Taken Up and Trafficked Via Different Metabolic Pathways in Porcine Adipocytes

  • Original Article
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Lipids

Abstract

Dietary fatty acids have different effects on fat deposition in pigs. To clarify the underlying mechanisms of this difference, we compared the metabolism of palmitic (PAM, saturated), oleic (OLA, monounsaturated) and linoleic acid (LNA, polyunsaturated) in porcine adipocytes treated with 100 μM PAM, OLA or LNA. We observed that the adipocytes incubated with LNA accumulated more lipids compared with those treated with PAM and OLA. We then probed the metabolism of these fatty acids in porcine adipocytes by using isotope-labelled fatty acids. The results showed that 42% of the [1-14C] LNA, 34% of the [1-14C] PAM and 28% of the [1-14C] OLA were recovered in the cellular lipids. The gene expression analyses showed that LNA significantly increased the expression of adipogenesis- and oxidation-related genes including PPARγ, C/EBPα, ap2 and NRF1. In addition, the cells incubated with LNA showed a decreased Ser112 phosphorylation in PPARγ compared to those incubated with PAM and OLA. Furthermore, when PPARγ Ser112 phosphorylation was inhibited, no significant difference in the triacylglycerol contents in the adipocytes was observed. These results showed the dietary fatty acids had different metabolism pathways in porcine adipocytes, and LNA significantly promoted lipid accumulation, probably by regulating PPARγ phosphorylation in adipocytes.

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Abbreviations

ACSL1:

Acyl-CoA synthetase long-chain family member 1

aP2:

Fatty acid binding protein 4

ASP:

Acid-soluble products

CD36:

CD36 molecule

CE:

Cholesteryl esters

C/EBPα:

CCAAT/enhancer-binding proteins α

COX:

Cytochrome C oxidase

CPT1:

Carnitine palmitoyltransferase 1

Cyt C:

Cytochrome C

DAG:

Diacylglycerol

FBS:

Fetal bovine serum

FA:

Fatty acid

IBMX:

3-isobuty-1-methylxanthine

LNA:

Linoleic acid

LCFA:

Long chain FA

MAG:

Monoacylglycerol (s)

MAPK:

MAP kinase

NRF1:

Nuclear respiratory factor 1

OLA:

Oleic acid

PL:

Phospholipids

PAM:

Palmitic acid

PPARγ:

Peroxisome proliferator-activated receptor γ

SFA:

Saturated fatty acids

SREBP1:

Sterol response element–binding protein 1

STA:

Stearic acid

TAG:

Triacylglycerol

TLC:

Thin-layer chromatography

Ucp-2:

Uncoupling protein 2

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Authors and Affiliations

  1. Key Laboratory of Animal Nutrition and Feed Sciences, Ministry of Agriculture, Zhejiang Provincial Laboratory of Feed and Animal Nutrition, College of Animal Sciences, Zhejiang University, No. 866 Yuhangtang Road, Hangzhou, 310058, Zhejiang, People’s Republic of China

    Caihua Yu, Lingling Xi, Jin Chen, Qin Jiang, Hongbo Yi, Yizhen Wang & Xinxia Wang

Authors
  1. Caihua Yu
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  2. Lingling Xi
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  3. Jin Chen
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  4. Qin Jiang
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  5. Hongbo Yi
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  6. Yizhen Wang
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  7. Xinxia Wang
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Contributions

The author’s contributions were as follows: YW, XW and CY contributed to the study design. CY, XW and JC wrote the manuscript and carried out data analyses. QJ and LX help to carry out data analysis. HY helped to modify the manuscript. All authors read and approved the final version of the manuscript.

Corresponding author

Correspondence to Xinxia Wang.

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Funding

This work was supported by a Grant from National Basic Research Program of China (973 Program) (no. 2012CB124705) and the earmarked fund for Modern Agroindustry Technology Research System (CARS-36).

Conflict of interest

The authors declare there is no conflict of interest.

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Yu, C., Xi, L., Chen, J. et al. PAM, OLA, and LNA are Differentially Taken Up and Trafficked Via Different Metabolic Pathways in Porcine Adipocytes. Lipids 52, 929–938 (2017). https://doi.org/10.1007/s11745-017-4302-x

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  • DOI: https://doi.org/10.1007/s11745-017-4302-x

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