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Differential proteomic analysis of polyubiquitin-related proteins in chemical hybridization agent-induced wheat (Triticum aestivum L.) male sterility

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Abstract

Protein polyubiquitination is a significant regulator of diverse physiological functions, including sexual reproduction, in plants. Chemical hybridizing agents (CHA) SQ-1 has been shown to induce male sterility in wheat (Triticum aestivum L.) through inhibition of pollen development. This mechanism by which CHA induces male sterility in wheat is unclear. In this study, differential proteomic analysis of polyubiquitinated proteins associated with wheat male sterility was investigated. Wheat plants of the same genetic background were treated with or without CHA. Ubiquitinated proteins were then extracted and enriched for proteomic analysis. Differentially expressed polyubiquitinated proteins in trinuclear stage anther were identified by nanospray liquid chromatography/tandem mass spectrometry. A total of 127 and 131 differentially expressed polyubiquitinated proteins, including heat shock protein 70, ATPase subunit, glycosyltransferase, ubiquitin-related enzyme, and 20S proteasome subunit, were successfully identified by searching against wheat protein database and NCBInr database, respectively. Most of these proteins are related to photosynthesis, carbohydrate and energy metabolism, and multiple metabolic processes. These findings show that alteration of polyubiquitinated proteins is associated with male sterility in wheat.

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Abbreviations

CHA:

Chemical hybridizing agents

Ub:

Ubiquitin

ERAD:

Endoplasmic reticulum-associated degradation

UPP:

Ub/proteasome pathway

SCF:

SKP-cullin-F-box protein

nano-LC/MS/MS:

Nanospray liquid chromatography/tandem mass spectrometry

GT:

Glycosyltransferase

HSPs:

Heat shock proteins

SEM:

Scanning electron microscopy

GV:

Grayscale value

NW:

Normal wheat

PMS:

Physiologically male sterile

SDS-PAGE:

Sodium dodecyl sulfate–polyacrylamide gel electrophoresis

MBF:

MADS-box transcription factor

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Acknowledgments

This work was supported by National High Technology Research and Development Program of China (No. 2011AA10A106), National Natural Science Foundation of China (Nos. 31071477,31171611), the Research Fund for the Doctoral Program of Higher Education (No. 20090204110024), and the Key Scientific and Technological Innovation Special Projects of Shaanxi province “13115” (Nos. 2010ZDKG-68, 2011KTZB02-01-01).

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Authors and Affiliations

  1. National Yangling Agricultural Biotechnology and Breeding Center, Yangling Branch of State Wheat Improvement Centre, Wheat Breeding Engineering Research Center, Ministry of Education, Key Laboratory of Crop Heterosis of Shaanxi Province, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Hongzhan Liu, Gaisheng Zhang, Wanwan Zhu, Qingsong Ba, Longyu Zhang, Na Niu, Shoucai Ma & Junwei Wang

  2. The Chinese University of Hong Kong, Hong Kong, China

    William K. K. Wu & Lin Zhang

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  1. Hongzhan Liu
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  2. Gaisheng Zhang
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  5. Qingsong Ba
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  6. Lin Zhang
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  7. Longyu Zhang
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  8. Na Niu
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  9. Shoucai Ma
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  10. Junwei Wang
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Correspondence to Gaisheng Zhang.

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Communicated by Z.-L. Zhang.

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Liu, H., Zhang, G., Zhu, W. et al. Differential proteomic analysis of polyubiquitin-related proteins in chemical hybridization agent-induced wheat (Triticum aestivum L.) male sterility. Acta Physiol Plant 36, 1473–1489 (2014). https://doi.org/10.1007/s11738-014-1525-9

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  • DOI: https://doi.org/10.1007/s11738-014-1525-9

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